全自动微生物分析系统和16S rDNA序列分析技术对奶牛乳腺炎病原菌的鉴定

从四川成都、绵阳和眉山地区部分奶牛场共分离奶牛乳腺炎病原菌120株,采用VITEK全自动微生物分析系统和16S rDNA序列分析技术对分离菌株进行鉴定。研究发现引起四川成都、绵阳和眉山奶牛乳腺炎以细菌混合感染为主,主要致病菌有葡萄球菌、大肠杆菌、单胞菌属、鲁氏不动杆菌、吉氏库特氏菌和芽孢杆菌属。其中,葡萄球菌的感染最为普遍,检出率最高(33株,27.50%);其次为大肠杆菌(17株,14.17%)、单胞菌属(12株,10%)、鲁氏不动杆菌(11株,9.17%)、吉氏库特氏菌(9株,7.50%)和芽孢杆菌属(9株,7.50%);再次为巨型球菌(6株,5%)、阴沟肠杆菌(6株,5.00%)、产吲哚金黄杆菌(6株,5.00%)和链球菌属(4株,3.33%);最低的是棒杆菌属(3株,2.50%)、粪肠球菌(2株,1.67%)、弗氏志贺菌(1株,0.83%)和普通变形杆菌(1株,0.83%)。本研究为防治奶牛乳腺炎奠定了基础。

Application of VITEK 32 microbial analytical system and sequence analysis of 16S rDNA in identification of pathogenic bacteria from dairy cow with mastitis

Pathogenic bacteria were isolated from dairy cow with mastitis in Chengdu,Meishan and Mianyang,and 120 bacterial strains were obtained.The bacteria were identified by methods of microbial analytical system and sequence analysis of 16S rDNA.The results showed that there were 33 strains of staphylococcus(27.5%),17 of E.coli(14.17%),12 of Aeromonas(10.00%),11 of Presumptive acinetobacter lwoffi(9.17%),9 of Lurthia gibsonii(7.50%),9 of bacillus(7.50%),6 of Macrococcus caseolyticus(5.00%),6 of Enterobacterium casei(5.00%),6 of Chrvseobacterium indologenes(5.00%),4 of Streptococcus(3.33%),3 of Corynebacterium(2.50%),2 of Enterococcus faecalis(1.67%),1 of Shigella flexneri(0.83%) and 1 of Proteus vulgaris(0.83%).This study provides a basis for the control of bovine mastitis.