| STAT5b基因的遗传多态性及其与京海黄鸡体重和繁殖性状的关联分析 |
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| 引用本文： | 赵秀华,王金玉,张跟喜,金崇富,顾玉萍,俞亚波,施会强.STAT5b基因的遗传多态性及其与京海黄鸡体重和繁殖性状的关联分析[J].畜牧与兽医,2011,43(8). |
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| 作者姓名： | 赵秀华 王金玉 张跟喜 金崇富 顾玉萍 俞亚波 施会强 |
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| 作者单位： | 1. 扬州大学动物科学与技术学院,江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009
2. 江苏京海集团,江苏南通,226103 |
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| 基金项目： | 国家肉鸡产业技术体系资助(nycytx-42-G1-05) |
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| 摘 要： | 以京海黄鸡、AA鸡、尤溪麻鸡、边鸡等4个鸡品种为研究对象,采用PCR-SSCP方法检测信号转导和转录激活子5b(STAT5b)第7外显子和部分内含子7的多态性,并分析其对京海黄鸡体重和繁殖性状的遗传效应。结果显示,在STAT5b第7内含子区域检测到1处突变(8 066bp C→T)。在4个鸡品种中均检测到AA、AB、BB 3种基因型,χ2检验结果表明,除京海黄鸡外其余3个品种群体在该座位均达到Hardy-Weinberg平衡(P>0.05)。最小二乘分析表明,京海黄鸡3种基因型个体的出生重和开产日龄存在显著差异(P<0.05)。
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| 关 键 词： | 京海黄鸡 信号转导和转录激活子5b基因(STAT5b) 遗传多态性 体重 繁殖性状 |
Genetic polymorphisms of the STAT5b gene and its relationship with body weights and reproduction traits in Jinghai chicken |
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| ZHAO Xiu-hua,WANG Jin-yu,ZHANG Gen-xi,JIN Chong-fu,GU Yu-ping,YU Ya-bo,SHI Hui-qiang.Genetic polymorphisms of the STAT5b gene and its relationship with body weights and reproduction traits in Jinghai chicken[J].Animal Husbandry & Veterinary Medicine,2011,43(8). |
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| Authors: | ZHAO Xiu-hua WANG Jin-yu ZHANG Gen-xi JIN Chong-fu GU Yu-ping YU Ya-bo SHI Hui-qiang |
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| Institution: | ZHAO Xiu-hua1,WANG Jin-yu1,ZHANG Gen-xi1,JIN Chong-fu1,GU Yu-ping2,YU Ya-bo2,SHI Hui-qiang2(1.College of Animal Science and Technology,Yangzhou University,Key Laboratory for Animal Genetics,Breeding,Reproduction and Molecular Design of Jiangsu Province,Yangzhou 225009,China,2.Jiangsu Jinghai Poultry Group Co,Ltd,Nantong 226103,China) |
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| Abstract: | Single nucleotide polymorphisms of exon 7 and parts of intron 7 of the chicken STAT5b gene were detected in Jinghai,Arbor Acre,Bian and Youxi chickens by PCR-SSCP.Genetic effects of the STAT5b gene on body weights and reproduction traits of Jinghai chicken were analyzed.Results showed that there was one mutation in the region of intron 7 of STAT5b gene(8 066 bp C→T).For primer P1,three genotypes(AA,AB and BB) were detected in four chicken populations.The results of χ2 test showed that the frequencies of STAT5b gene fited with Hardy-Weinberg equilibrium in 3 breeds except Jinghai chicken(P>0.05).The least square means analysis showed there were significant difference among hatch weights,ages at first egg and genotypes,respectively(P<0.05). |
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| Keywords: | Jinghai chicken STAT5b gene genetic polymorphism body weight reproduction traits |
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