宁夏优质水稻品种D10高效再生体系的建立

为了利用基因沉默技术定向改良宁夏香稻品种，短期内建立可获得成苗的高效成熟胚再生体系，以宁夏优质水稻品种D10成熟胚作为外植体，通过植物组织培养的方法，研究了培养基、外源激素、光照和温度对外植体培养及再生的影响。结果表明：不同培养基对愈伤组织诱导率有着不同的影响，MS培养基最低，N6D培养基最高；32℃持续光照培养的愈伤组织明显优于30℃ 12 h/d光照培养；在分化培养基中添加TDZ，发现浓度为1 mg/L的TDZ与低浓度的IAA结合使绿苗分化率提高到100%；另外添加2 mg/L ABA的N6D培养基中愈伤组织诱导率为100%。最终该品种的最优再生体系为：32℃持续光照培养并添加ABA 2 mg/L的N6D培养基；分化培养基为B5+TDZ 1mg/L+IAA 0.2mg/L；生根培养基为1/2MS+KT 2 mg/L+NAA 0.2 mg/L+CH 2 g/L+Sorb 30 g/L。本研究建立了宁夏优质水稻品种D10成熟胚再生体系。

The Establishment of High Efficient Regeneration System in Ningxia Rice D10

The author choose the mature embryo of Ningxia high quality rice D10 as explants in tissue culture,in order to improving Ningxia fragrance rice with gene silence technology and established optimal and high efficient regeneration system. The effect of medium,exogenous plant hormone,genotype,light and temperature on vitro culture and regeneration system was studied by plant tissue culture method. The result showed that,different medium had different induction rate in D10. N6D medium was the highest induction rate,while MS medium was the lowest. The callus in 32℃ discontinued lighting culture condition were obviously better than30℃ 12 h/d. 1 mg/L TDZ combining with low concentration IAA improved green shoot induction rate to 100%.Additionally,N6D medium pulsed 2 mg/L ABA could increased callus induction rate to 100%. The optimum condition and medium were 32℃ discontinued lighting culture in N6D medium pulsed 2 mg/L ABA,B5＋TDZ1 mg/L ＋IAA 0.2 mg/L differentiation medium and 1/2MS＋KT 2 mg/L＋NAA 0.2 mg/L＋CH 2 g/L＋Sorb 30 g/L shooting medium. The high efficient regeneration system in mature embryo of Ningxia high quality rice D10was established.