| 无芒隐子草CsLEA基因超表达载体和反义表达载体构建 |
| |
| 引用本文： | 段珍, 狄红艳, 张吉宇, 霍雅馨, 孔令芳. 无芒隐子草CsLEA基因超表达载体和反义表达载体构建[J]. 草业科学, 2014, 8(8): 1475-1480. DOI: 10.11829/j.issn.1001-0629.2013-0458 |
| |
| 作者姓名： | 段珍 狄红艳 张吉宇 霍雅馨 孔令芳 |
| |
| 作者单位： | 1.草地农业生态系统国家重点实验室 兰州大学草地农业科技学院, 甘肃 兰州 730020 |
| |
| 摘 要： | 根据已克隆出的无芒隐子草(Cleistogenes songorica)晚期胚胎发生丰富蛋白基因CsLEA(GenBank序列号为FJ972827)基因和植物表达载体的酶切位点特征, 分别将CsLEA基因编码区序列480 bp正向和反向插入pBI121, 构建了超表达载体pBI121 35S::CsLEA和反义表达载体pBI121 35S::AS-CsLEA。电击法转入根癌农杆菌GV3101中, 并通过花粉管通道法转化拟南芥(Arabidopsis thaliana), 经卡那霉素抗性筛选和PCR鉴定, 已获得T1代阳性植株。
|
| 关 键 词： | CsLEA基因 超表达载体 反义表达载体 拟南芥 |
| 收稿时间： | 2013-08-14 |
Construction of the over and antisense expression vector of CsLEA gene |
| |
| Zhen DUAN, Hong-yan DI, Ji-yu ZHANG, Ya-xin HUO, Ling-fang KONG. Construction of the over and antisense expression vector of CsLEA gene[J]. Pratacultural Science, 2014, 8(8): 1475-1480. DOI: 10.11829/j.issn.1001-0629.2013-0458 |
| |
| Authors: | Zhen DUAN Hong-yan DI Ji-yu ZHANG Ya-xin HUO Ling-fang KONG |
| |
| Affiliation: | 1.State Key Laboratory of Graaland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China |
| |
| Abstract: | According to the restriction enzyme sites of plant expression vector and sequence of CsLEA gene from Cleistogenes songorica, over expression vector was recombined by inserting the open reading frame (ORF) sequence to pBI121, and the antisense plant expression vector was constructed by ligating 480 bp sequence of CsLEA gene to pBI121 in the antisense orientation. Moreover, the recombined plasmid was transformed into Agrobacterium tumefaciens GV3101 by electroporation method. Using pollen-tube pathway, the plasmid was transformed into Arabidopsis thaliana and T1 positive plants were obtained by kanamycin resistance selection and PCR confirmation. |
| |
| Keywords: | CsLEA gene over expression vector antisense expression vector Arabidopisis thaliana |
|
| 点击此处可从《草业科学》浏览原始摘要信息 |
|
点击此处可从《草业科学》下载全文 |
|
