动物源血液中三聚氰胺的液相色谱质谱联用检测方法的建立

为建立不同动物源血液中三聚氰胺的质谱联用检测方法,通过研究不同提取溶剂提取效率和不同固相萃取柱净化能力,进行回收率、精密度和检出限等技术要素评价。分析得到,以甲醇:水:乙酸溶液(49.5:49.5:1)作为提取试剂,MCX柱作为净化固相萃取柱,各种动物源血液样品中三聚氰胺回收率为90.0%~110%,精密度RSD为0.96%~4.64%,方法定量限为10μg·kg~(-1)。试验表明此方法灵敏度高,稳定性好,适用于动物血液样品中三聚氰胺有机物含量的检测。

The establishment of a detection method for detecting melamine in animal-derived blood

In order to establish a Mass spectrometry method for determination of melamine in animal-derived blood,we investigated the extraction efficiency of various solvents and the purification ability of different SPE columns and evaluated the technical parameters including recovery rate,precision and detection limit.With the 49.5:49.5:1 (methanol:water:acetic acid) solution as an extraction reagent and using MCX column as purifying solid-phase extraction column,the melamine in animal blood samples of recovery ranged from 90.0％ to 110.0％;the precision RSD was in the range of 0.96 ％-4.64％;and the detection limit was 10 μg.kg-1.The result indicated that this optimized method was sensitive and stable,which could be potentially applied to determination of melamine in animal blood samples.