| 柑桔合子胚早期离体培养获得植株 |
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| 引用本文： | 陈振光,王家福.柑桔合子胚早期离体培养获得植株[J].福建农林大学学报(自然科学版),1986(4). |
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| 作者姓名： | 陈振光 王家福 |
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| 作者单位： | 福建农学院亚热带果树研究所，福建农学院亚热带果树研究所 |
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| 摘 要： | 通过柑桔胚胎发育观察表明,授粉后50天左右,胚囊内仅有一个合子胚,未见珠心胚侵入胚囊,为单独分离合子胚培养的最好时期。取这一发育期的幼果,分离合子胚(或将胚囊预培养后分离合子胚),接种于 White 基本培养基附加腺嘌呤(Ad)20毫克/升、水解乳蛋白(LH)400毫克/升、椰乳(CM)10%和蔗糖10%,在光照条件下发育成双子叶胚。转入 MT 基本培养基附加玉米素(Ze)0.25毫克/升、赤霉素(GA_3)5～10毫克/升,蔗糖浓度降至2%,便发育成苗。
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| 关 键 词： | 柑桔 合子胚 珠心胚 离体培养 |
INDUCTION OF CITRUS PLANTS FROM ZYGOTIC EMBRYOS IN VITRO CULTURE AT THE EARLY STAGE OF EMBRYO DEVELOPMENT |
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| Chen Zhenguang and Wang Jiafu Research Institute of Subtropical Pomology,FAC.INDUCTION OF CITRUS PLANTS FROM ZYGOTIC EMBRYOS IN VITRO CULTURE AT THE EARLY STAGE OF EMBRYO DEVELOPMENT[J].Journal of Fujian Agricultural and Forestry University,1986(4). |
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| Authors: | Chen Zhenguang and Wang Jiafu Research Institute of Subtropical Pomology FAC |
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| Abstract: | Observation of citrus embryo development showed that in 50 days or so after pollination there was only one zygotic embryo in the embryo sac, and nucellar embryo was not yet invaded.This was the best time of the zygotic embryo to separate and culture.The young fruits at this stage were picked,and the zygotic embryos were separated(or after pre-culture), inoculated on the basic medium White,supplemented with Ad(20mg/L), LH (400mg/L),CM(10%)and sucrose(10%),and developed into dico- tyledonous embryos under artifical illumination.The dicotyledonous embryos were again transferred to the basic medium MT supplemented with Ze (0.25mg/L),GA_3(5-10mg/L)and sucrose(2%),where they deve- loped into seedlings. |
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| Keywords: | citrus zygotic embryo nucellar embryo in vitro culture |
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