富铁苹果发酵液对缺铁胁迫下平邑甜茶保护酶活性的影响

以缺铁平邑甜茶(M.hupehensis Rehd)为试材，用富铁苹果发酵液进行恢复试验。结果表明：对一年生平邑甜茶幼苗，T3处理（稀释300倍发酵液+FeSO4）较T1处理（去离子水对照）明显提高了平邑甜茶幼苗CAT、POD的活性和MDA、可溶性糖含量，且T2处理（稀释100倍发酵液+FeSO4）比T4处理（稀释500倍发酵液+FeSO4）作用明显；对于三年生缺铁苹果树来说，A3（发酵液+ FeSO4）处理较A1（浇去离子水对照）、A2（FeSO4 溶液）处理明显提高了CAT、POD的活性和MDA、可溶性糖的含量。 总体上，稀释300倍发酵液+FeSO4效果最好。

Study on the Effect of Iron-rich Apple Fruit broth on Protective Enzymes Activities of iron-deficiency Malus hupehensis

To study the effects of different fold diluted apple fermentation broth＋FeSO4 on protective enzymes activities of iron-deficiencyMalus hupehensis. Iron-deficiencyMalus hupehensis seedlings cultured in sand were watered with the iron-rich apple fermentation broth to recover the iron-deficiency phenomenon. The results showed that： For Malus hupehensis seedlings, compared with T1 （deionized water） treatment, the activities of CAT, POD and contents of MDA, soluble sugar of T3 （diluted 300-fold fermentation broth ＋FeSO4） treatment increased 283.6%, 58.2%, 271.8%, 50.3%, respectively. The four indexes of T3 treatment was also higher than the other four treatments significantly. Except the soluble sugar, the effect of T2 （diluted 100-fold fermentation broth ＋FeSO4） treatment was better than T4 （diluted 500-fold fermentation broth ＋FeSO4） and T5 （FeSO4） treatment significantly. For three-years iron-deficiency apples, compared with A1 （deionized water） treatment, the activities of CAT, POD and contents of MDA, soluble sugar of A3（diluted 300-fold fermentation broth ＋FeSO4） treatment increased 400.0%, 88.3%, 39.4%, 48.4% respectively. The effect of A3 （diluted 300-fold fermentation broth ＋FeSO4） was better than A2 （FeSO4） treatment significantly. As a whole, 300-fold diluted fermentation broth ＋FeSO4 was the best treatment for both iron-deficiency seedings and three-years iron-deficiency apples.