| 芸薹属查尔酮异构酶基因家族RNA干扰载体的构建 |
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| 引用本文： | 曹廷,冯瑜,牛海霞,柴友荣.芸薹属查尔酮异构酶基因家族RNA干扰载体的构建[J].广西农业生物科学,2010(5). |
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| 作者姓名： | 曹廷 冯瑜 牛海霞 柴友荣 |
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| 作者单位： | [1]西南大学农学与生物科技学院 [2]农业部生物技术与作物品质改良重点实验室 [3]重庆市作物品质改良重点实验室 [4]重庆市油菜工程技术研究中心 |
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| 基金项目： | 国家“863”计划(2008AA10Z152;2006AA10Z110);; 重庆市科技攻关项目(CSTC2009AB1030)共同资助 |
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| 摘 要： | 作为类黄酮途径的第二步关键酶,查尔酮异构酶(chalcone isomerase,CHI)催化四羟基查尔酮成为柚皮素,用于合成各种类黄酮物质,影响多种性状。芸薹属含有多种重要的油料、蔬菜和观赏植物。本研究从甘蓝型油菜克隆了芸薹属CHI基因家族的干扰片段BCHII(761bp),采用NcoⅠ+AatⅡ和BamHⅠ+XbaⅠ双酶切方案分别将其反义片段和正义片段亚克隆到植物RNA干扰(RNAi)平台载体pFGC5941M的启动子与间隔区、间隔区与终止子之间,形成了11674bp的RNAi载体pFGC5941M-BCHII(简称pBCHII),多种PCR检测表明2个片段的插入方向正确,重组载体完整,将其转化根癌农杆菌菌株LBA4404以后获得了工程菌株LBA4404-pBCHII-①。本研究结果将促进研究CHI与芸薹属异花授粉、植株色彩、黄籽和抗逆性等性状的关系和开展相关分子育种。
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| 关 键 词： | 芸薹属 类黄酮 查尔酮异构酶（CHI） RNA干扰 载体构建 |
Construction of a RNA Interference Vector of Brassica Chalcone Isomerase Gene Family |
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| Cao Ting Feng Yu Niu Haixia Chai Yourong.Construction of a RNA Interference Vector of Brassica Chalcone Isomerase Gene Family[J].Journal of Guangxi Agricultural and Biological Science,2010(5). |
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| Authors: | Cao Ting Feng Yu Niu Haixia Chai Yourong |
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| Institution: | Cao Ting Feng Yu Niu Haixia Chai Yourong Chongqing Rapeseed Technology Research Center; Chongqing Key Laboratory of Crop Quality Improvement; Key Lab of Biotechnology & Crop Quality Improvement of Ministry of Agriculture; College of Agronomy and Biotechnology; Southwest University; Chongqing; 400716; |
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| Abstract: | As the second-step key enzyme of flavonoid pathway, chalcone isomerase (CHI) catalyzes naringenin chalcone into (2S)-naringenin, which is used to synthesize various flavonoids, influencing multiple traits. Brassica contains many important oilseed, vegetable and ornamental crops. In this study, the 761 bp Brassica CHI gene family RNA interference (RNAi) fragment BCHII from B. napus was cloned, a 11 674 bp RNAi vector pFGC5941MBCHII (simplified as pBCHII) was constructed by subcloning its sense and antisense ... |
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| Keywords: | Brassica Flavonoids Chalcone isomerase （CHI） RNA interference Vector construction |
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