外源甲醇对棉花乙醇酸氧化酶活性和光合速率的影响

棉花(新陆早36)生殖生长期喷施外源甲醇水溶液后,检测分析了叶片中乙醇酸氧化酶活性、光合速率及籽棉产量。结果表明,不同生长期,甲醇水溶液处理后对乙醇酸氧化酶活性的抑制作用强弱表现为:盛蕾期>盛花期>铃期;对乙醇酸氧化酶活性的抑制作用为:30%甲醇水溶液>20%甲醇水溶液>10%甲醇水溶液。当处理组叶片乙醇酸氧化酶活性受到不同程度抑制时,光合速率相应提高,籽棉产量亦增高。试验结果表明,高光强、高温情况下喷施外源甲醇水溶液增强光合作用效率,与其参与棉花作物的光呼吸代谢有关。

Effects of Methanol on Glycolate Oxidase and Photosynthetic Rate of Cotton

In this experiment,10%,20% and 30% aqueous methanol were sprayed on foliage of cotton plants in arid environments under reproductive stage,and the activity of glycolate oxidase(GLO),photosynthetic rate,unginned yield were mensurated.The results showed that after spraying aqueous methanol,the activity of GLO dropped significantly and sustainably.And this inhibitory action in the full-bud stage is greater than that in the full-bloom stage and the boll stage,the aqueous methanol of 30% is better than that of 20% and 10%.The tested photosynthetic rate in the same period showed that there is a negative relation between photosynthetic rate and activity of GLO.When the activity of leaves Glycolate oxidase were inhibited,the photosynthetic rate was increased correspondingly,the yield of cotton plants and yield of cotton seeds were also increased.The results also indicated that the methanol may involve in that metabolism of photorespiration by spraying aqueous methanol on cotton in bright sunlight and high temperature conditions.