| 牛LPL基因的遗传变异与肉品质性状的关联分析 |
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| 引用本文： | 郭艳青,许尚忠,孙宝忠,高雪,任红艳,陈金宝.牛LPL基因的遗传变异与肉品质性状的关联分析[J].农业生物技术学报,2007,15(5):899-900. |
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| 作者姓名： | 郭艳青 许尚忠 孙宝忠 高雪 任红艳 陈金宝 |
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| 作者单位： | 中国农业科学院畜牧研究所,北京,100094 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 脂蛋白脂肪酶(lipoprotein lipase，LPL)是脂肪代谢过程中的关键酶，LPL基因被确定为影响相关肉质性状的重要候选基因。本研究利用PCR-RFLP技术对安格斯牛、海福特牛、中国西门塔尔牛、夏洛来牛、鲁西牛和西门塔尔×蒙古牛杂交牛6个品种的238头个体LPL基因进行多态性分析。结果表明，扩增片段大小为378bp的片段存在MspⅠ酶切多态性，263bp处发生A/G突变。 实验中所有牛品种在该基因座位都处于Hardy-Weinberg平衡状态(P>0.05)。除安格斯牛外，其它群体均处于中度多态（0.250.05）
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| 关 键 词： | 牛 LPL基因 PCR-RFLP 背膘厚 |
| 文章编号： | 1006-1304（2007）05-0899-02 |
| 收稿时间： | 2006-09-27 |
| 修稿时间： | 2006-11-14 |
Genetic Variation of LPL Gene and Association Analysis with Meat Quality Traits in Bovine |
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| GUO Yan-qing,XU Shang-zhong,SUN Bao-zhong,GAO Xue,REN Hong-yan,CHEN Jin-bao.Genetic Variation of LPL Gene and Association Analysis with Meat Quality Traits in Bovine[J].Journal of Agricultural Biotechnology,2007,15(5):899-900. |
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| Authors: | GUO Yan-qing XU Shang-zhong SUN Bao-zhong GAO Xue REN Hong-yan CHEN Jin-bao |
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| Institution: | Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China |
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| Abstract: | Lipoprotein lipase（LPL）is a key enzyme for the metabolize of circulating triacylglycerols. It is considered to be the major candidate gene affecting fat thickness and intramuscular fat content etc. In this study, PCR-RFLP was applied to analyze the polymorphism of LPL gene in 238 cattle that comprised of six bovine breeds, namely Angus、Hereford、Chinese simmental、Charolais、 Luxi cattle、simmental×Mongolia cattle. A 378 bp-long PCR product was digested with MspⅠ. The result showed that BB genotype had one substitution mutation A/G in 263bp. All six breeds were at Hardy-Weinberg equilibrium（P>0.05）.Except Angus, the polymorphism information contents of other breeds were moderate polymorphisms（0.25
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| Keywords: | PCR-RFLP |
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