Cross-reactivity of anti-Taenia crassiceps cysticerci immune antibodies with Taenia solium antigens |
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| Authors: | Espíndola N M De Gaspari E N Nakamura P M Vaz A J |
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| Affiliation: | Faculty of Pharmaceutical Sciences, University of S?o Paulo, Av Prof. Lineu Prestes 580, Bloco 17, 05508-900, S?o Paulo, Brazil. |
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| Abstract: | A comparative study of antibody production was carried out using BALB/c mice immunized with 20 or 50microg vesicular fluid (VF)-Tcra (Taenia crassiceps) antigens, and gel of <30kD or eluate from <30kD peptides. Good IgM, IgA and IgG levels were detected by ELISA-Tcra and the antibodies presented reactivity with the <20kD peptides when tested by immunoblotting-Tcra. The antibodies from animals immunized with 20 and 50microg presented high anti-Tso cross-reactivity in ELISA (IgG>IgM and IgA). All groups presented IgG antibodies identifying the 12kD Tso-peptide. |
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