草莓枯萎病菌抗戊唑醇突变体ZY-W的生理生化特性

为明确抗戊唑醇草莓枯萎病菌 Fusarium oxysporum f.sp. fragariae 突变体ZY-W的生理生化特性,分析其抗性风险及可能的抗性机制,比较了对戊唑醇抗性达34.2倍的ZY-W和敏感菌株ZY在苯丙氨酸解氨酶(PAL)及过氧化物酶(POD)活力、对渗透压的敏感性、相对渗率、可溶性蛋白含量及酯酶同工酶图谱等方面的差异。结果表明:ZY-W突变菌株体内的POD和PAL活力均高于ZY;经不同浓度药剂处理0～24 h,2菌株PAL活力均呈先上升后下降的趋势,并在处理后1.5 h达到最高值;2菌株的POD活力则一直处于上升状态,24 h时达到最高值,且此时2菌株间POD活力差异最大;仅用清水处理0～24 h,2菌株的PAL和POD活力均一直处于上升状态。在不同处理时间及药剂浓度下,ZY的相对渗率为ZY-W的1.0～2.4倍,表明ZY-W菌株的细胞膜透性低于ZY;ZY-W对渗透压的敏感性及体内可溶性蛋白含量均显著高于ZY,其中ZY-W的可溶性蛋白含量是ZY的1.3倍;在突变菌株ZY-W的酯酶同工酶图谱中发现了 Rf值为0.24的特征性谱带。表明草莓枯萎病菌对戊唑醇产生抗性的同时,伴随着其生理生化特性的改变。

Physiological and biochemical characteristics of tebuconazole-resistant isolate of Fusarium oxysporum ZY-W

To determine the physiological and biochemical characteristics of tebuconazole-resistant isolate of Fusarium oxysporum f.sp. fragariae ZY-W,and calculate the resistance risk and mechanism,comparison was conducted between the resistant isolate ZY-W and the sensitive isolate ZY on the activity of phenylalanine ammonia lyase (PAL) and peroxidase (POD),the osmolarity sensitivity,the relative leakage,solution protein contents and electrophoretic patterns of esterases.The result showed that the activity of POD and PAL of ZY-W was higher than that of ZY at all time.The value of PAL treated by fungicide for 0-24 h was increased firstly and then decreased,and reached the peak at 1.5 h. The value of POD in the two isolates was rising all the time,and reached the top at the 24th hour,and the difference between two isolates reached the top at that time.The activity of PAL and POD in both isolates was rising after treated by double distilled water for 0-24 h.Under different conditions,ZY could leak more electrolyte than ZY-W,which means that cell membrane permeability of ZY-W is relatively low.Differences were also found in osmolarity and soluble protein content between two isolates,and the soluble protein content of ZY-W was 0.3 folds higher than that of ZY.In the electrophoretic patterns of esterases,one characteristic band was found in ZY-W.As the result showed,the development of fungicide resistance was accompanied by the changes of the physiology and biochemistry characteristics.