| 金焰笛鲷rDNA基因转录间隔区ITS-1序列分析 |
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| 引用本文： | 徐田军,刘楚吾,刘丽,吴勇.金焰笛鲷rDNA基因转录间隔区ITS-1序列分析[J].南方水产,2006,2(5):61-64. |
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| 作者姓名： | 徐田军 刘楚吾 刘丽 吴勇 |
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| 作者单位： | 广东海洋大学海洋生物研究所,广东,湛江,524025 |
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| 基金项目： | 广东省重大科技兴海项目(A200099A01) |
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| 摘 要： | 以持异性引物扩增了金焰笛鲷(Lutjamts fulviflamma)的核糖体第一转录间隔区(ITS-1),扩增产物经克隆后测序,测得 ITS-1长度为566 bp。其中 A、T、G、C 4种碱基的含量分别为14.1%、16.1%、30.2%、39.6%,G C(69.8%)含量明显高于 A T 含量(30.2%)。将此引物在笛鲷属其他4种鱼类中扩增,发现该对引物有很好的通用性;比较发现在不同种中 ITS-1存在着较大的差异,适合将其应用于分子系统学和种质资源方面的研究。
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| 关 键 词： | 金焰笛鲷 内转录间隔区(ITS-1) 序列分析 |
| 文章编号： | 1673-2227-(2006)05-0061-04 |
| 修稿时间： | 2006年6月3日 |
Sequence analysis of the internal transcribed spacer 1 (ITS-1) of ribosomal DNA gene of Lutjanus fulviflamma |
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| XU Tianjun,LIU Chuwu,LIU Li,WU Yong.Sequence analysis of the internal transcribed spacer 1 (ITS-1) of ribosomal DNA gene of Lutjanus fulviflamma[J].South China Fisheries Science,2006,2(5):61-64. |
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| Authors: | XU Tianjun LIU Chuwu LIU Li WU Yong |
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| Abstract: | The PCR technique was used to amplify rDNA-ITS-1 of Lutjanus fulviflamma,then the purified PCR productions were cloned into T-vector and sequenced by M13 /-primers.As a result,566 bp nucleotide sequences of rDNA-ITS1 were obtained.The average contents of A,T,G and C were 14.1%,16.1%,30.2% and 39.6%,respectively,the contents of GC(69.8%)were ob- viously higher than those of AT.Using these primers,ITS-1 region can be amplified in other four Lutjanus species,but the lengths of ITS-1 were largely different between different species. |
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| Keywords: | Lutjanus fulviflamma ITS-1 sequence analysis |
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