早期断奶对仔猪空肠和回肠兴奋性氨基酸载体1表达的影响

本试验旨在研究仔猪出生后10~20 d,早期断奶仔猪小肠谷氨酸转运载体基因表达情况与哺乳仔猪的差异。试验分别从40头不同母猪的仔猪中各选出体重相近,10日龄的"杜×长×大"三元杂交仔猪1头,共40头仔猪,随机不配对分为2组,每组20头仔猪,对照组(哺乳组)为哺乳仔猪,随母猪喂养;试验组(断奶组)为断奶仔猪,隔离断奶饲养;试验期10 d。饲养结束,每组随机取12只仔猪,宰杀取空肠和回肠,测定谷氨酸转运载体兴奋性氨基酸转运载体1(EAAC1)蛋白质表达情况和游离氨基酸含量。结果显示,断奶显著降低了仔猪空肠和回肠EAAC1(57和73 ku)及其相关蛋白谷氨酸转运联合蛋白(GTRAP3-18)(50 ku)的蛋白质和mRNA表达量(P0.05)。断奶提高了仔猪空肠游离谷氨酸和总氨基酸含量,却降低了仔猪回肠游离谷氨酸和总氨基酸含量,差异显著(P0.05)。结果提示,早期断奶降低EAAC1和GTRAP3-18的蛋白质含量,这可能与早期断奶仔猪遭受营养谷氨酸缺乏导致的肠道氨基酸吸收转运障碍有关。

Effects of Early Weaning on Jenunal and Ileal Excitary Amino Acid Aarrier 1 Expression of Piglets

This study was conducted to examine the effects of early weaning on adaptive changes in glutamate transporter gene expression in comparison with the suckling counterpart in the pig during the ages of 10 to 20 days.A total of 40 ternary hybrid piglets, with a similar body weight randomly taken from 40 different sows at 10 days of age, were divided into 2 groups.The trial group were 20 suckling piglets randomly obtained from 20 different sow litters, were allowed to continue suckling with their sows as the suckling group for 10 days;the 20 piglets of the weaning group were taken from another 20 different sow litters, moved off-site and weaned on a weaning diet for 10 days according to standard swine industry early weaning practices.At the end of the trial, 12 piglets were randomly taken from each group to remove the jejunum and ileum, and gene ex-pression of excitatory amino acid carrier 1 ( EAAC1) and free amino acids content were determined.The results showed that weaning significantly decreased the abundance of the EAAC1 ( 57 and 73 ku) and glutamate trans-porter associate protein 3-18 ( GTRAP3-18) ( 50 ku) protein and mRNA expression in jejunum and ileum of piglets ( P<0.05) .Weaning significantly increased the contents of glutamate and total free amino acids ( P<0.05) , while the contents of glutamate and total free amino acids in ileum were significantly decreased ( P<0.05).In conclusion, early weaning decrease jejunal and ileal EAAC1 and GTRAP3-18 protein expression and this may, in part, is related to early weaned piglets suffered from lack of nutrition leading to intestinal gluta-mate uptake disorder.