| 巴西橡胶树茉莉酸诱导胶乳cDNA文库的构建 |
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| 引用本文： | 聂智毅,李先昆,段翠芳,黎瑜.巴西橡胶树茉莉酸诱导胶乳cDNA文库的构建[J].中国农学通报,2010,26(13):376-379. |
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| 作者姓名： | 聂智毅 李先昆 段翠芳 黎瑜 |
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| 作者单位： | 中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,省部共建国家重点实验室培养基地-海南省热带作物栽培生理学重点实验室,海南儋州,571737 |
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| 基金项目： | 国家自然科学基金;中国热带农业科学院基金项目;中国热带农业科学院橡胶研究所基本科研业务费专项资金 |
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| 摘 要： | 摘要：以巴西橡胶树热研7-33-97为材料，对橡胶树割线下方施用0.1%茉莉酸，采集胶乳，提取胶乳总RNA并纯化mRNA，构建巴西橡胶树茉莉酸诱导胶乳融合表达文库。检测表明，获得的初级文库滴度为1.9×106 pfu/ml，插入片段在0.5到5kb之间，平均大小为1kb左右；表明该文库质量合格，为今后克隆胶乳中的茉莉酸信号应答基因奠定基础。
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| 关 键 词： | 新农村 新农村 庭院绿化 模式 效益评价 人居环境 |
| 收稿时间： | 2010-02-03 |
| 修稿时间： | 2010-03-05 |
Construction of jasmonic acid -induced expressed cDNA Library of latex in Hevea Brasiliensis |
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| Nie Zhiyi,Li Yu,Li Xiankun,Duan Cuifang.Construction of jasmonic acid -induced expressed cDNA Library of latex in Hevea Brasiliensis[J].Chinese Agricultural Science Bulletin,2010,26(13):376-379. |
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| Authors: | Nie Zhiyi Li Yu Li Xiankun Duan Cuifang |
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| Institution: | (Rubber Research Institute of CATAS, Key Laboratory of Rubber Biology of Ministry of Agriculture, State Key Laboratory Breeding Base of Cultivation ＆ Physiology of Tropical Crop, DanZhou Hainan 571737) |
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| Abstract: | Employing mRNA purified from the total RNA of latex in Clone 7-33-97 Hevea brasiliensis treated by 0.1% jasmonic acid （JA）, a JA-induced expressed eDNA library was constructed from latex in Hevea Brasiliensis. The titer of primary library is 1.9x 106 pfu/mL. The sizes of inserted fragments in the vector were between 500-5000bp, the average size was about 1 kb. These data indicates that this eDNA Library has a high quality, and therefore provides a useful tool for the cloning of JA responsive genes in latex of Hevea brasiliensis. |
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| Keywords: | Jasmonic acid Hevea brasiliensis zz cDNA libraryzz |
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