小麦ZY96-3籽粒灌浆过程中籽粒发育相关基因的表达分析

【目的】 研究小麦ZY96-3籽粒在灌浆过程中与籽粒发育相关基因的表达模式,为了解基因调控籽粒灌浆的分子机制提供参考。【方法】 采用实时荧光定量PCR(qRT-PCR)技术,分析小麦ZY96-3籽粒灌浆期间6个与籽粒发育相关基因的表达动态。【结果】 从小麦ZY96-3开花后6个目标基因的表达模式均是先增后降,且都在花后7 d开始表达;与粒重相关基因TaTGW6在花后7 d表达量最高,与籽粒大小相关基因TaCYP78A5、蛋白质合成基因TaPBF-D和淀粉合成酶基因GBSSⅠ、SBE1、AGPase1都在花后15 d左右表达量达到最大;自花后19 d开始,各基因的表达量均显著下降。【结论】 与粒重相关基因TaTGW6主要在小麦ZY96-3籽粒灌浆初期起关键作用,而其余5个基因主要在籽粒灌浆中后期发挥功能。基因在小麦ZY96-3籽粒灌浆期间的表达模式。

Expression of Grain Development Related Genes in Wheat ZY96-3 during Grain Filling Stage

【Objective】 　To explore the expression pattern of ZY96-3 genes related to grain development during grain filling stage. in order to provide reference for further understanding of the molecular mechanism of gene regulation in grain filling. 【Method】 The real-time fluorescence quantitative PCR (qRT-PCR) technique was used to analyze the expression dynamics of 6 genes related to grain development during grain filling stage of ZY96-3. 【Result】The results showed that the expression patterns of six target genes of ZY96-3 increased first and then decreased, and all of them began to express 7 days after anthesis; TaTGW6 genes were involved in grain weight on the 7 d after flowering to express the highest amount, and the grain size TaCYP78A5 related genes, protein synthesis genes TaPBF - D and starch synthase gene GBSS Ⅰ, SBE1, AGPase1 all around 15 d after flowers express maximum amount; However, the expression level of each gene decreased significantly at 19 d after anthesis.【Conclusion】 This study showed that the grain-weight related gene TaTGW6 mainly played a key role in the early stage of wheat ZY96-3 grain filling, while the other 5 genes mainly played a role in the middle and late stage of wheat grain filling.