| β-葡萄糖苷酶高产菌株的诱变选育及固体发酵条件的研究 |
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| 引用本文： | 杨建海,任大明.β-葡萄糖苷酶高产菌株的诱变选育及固体发酵条件的研究[J].安徽农业科学,2005,33(8):1566-1568. |
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| 作者姓名： | 杨建海 任大明 |
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| 作者单位： | 沈阳农业大学生物技术学院生物工程重点实验室,辽宁,沈阳,110161 |
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| 摘 要： | 以黑曲霉(Aspergillusniger)为出发菌株,经紫外线(UV)、硫酸二乙酯(DES)、60Co诱变,筛选出β-葡萄糖苷酶的活力比原菌株明显提高。并通过单因素和正交实验研究了该酶的固体发酵条件,采用优化配方,使酶活力达到169.2U/ml。
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| 关 键 词： | 黑曲霉 β-葡萄糖苷酶 诱变 固体发酵 |
| 文章编号： | 0517-6611（2005）08-1566-03 |
| 收稿时间： | 05 19 2005 12:00AM |
| 修稿时间： | 2005-05-19 |
Selection of High-yield β-glucosidase Strain and the Optimized of Solid State Fermentation |
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| Yang JianHai;Ren DaMing.Selection of High-yield β-glucosidase Strain and the Optimized of Solid State Fermentation[J].Journal of Anhui Agricultural Sciences,2005,33(8):1566-1568. |
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| Authors: | Yang JianHai;Ren DaMing |
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| Abstract: | The Aspergillus niger mutant strain was been selected by alternative treating Aspergillus niger with UV, EDS and 60Co. The enzymeactivityofβ-glucosidase fromfermentation ofthe mutant was heightened compared with the original. Further more the fermentation conditions forthe mutant were optimized through single factor and orthogonal experiments. Under the optimal condition, the enzyme activity of β-glucosidaseattained 169.2 U/ml. |
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| Keywords: | Aspergillus niger β- glucosidase Solid state fermentation Mutation |
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