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| TDZ诱导中间锦鸡儿植株再生 | |
| 引用本文： | 胡钠梅,韩素英,梁国鲁,齐力旺.TDZ诱导中间锦鸡儿植株再生[J].北方园艺,2009(8). |
| 作者姓名： | 胡钠梅 韩素英 梁国鲁 齐力旺 |
| 作者单位： | 1. 西南大学,园艺园林学院,重庆,400716;中国林科院林业研究所,细胞生物学实验室,北京,100091 2. 中国林科院林业研究所,细胞生物学实验室,北京,100091 3. 西南大学,园艺园林学院,重庆,400716 |
| 基金项目： | 国家“十一五”科技支撑资助项目(2006BAD01A16);;国家高科技研究发展计划资助项目(863计划)(2007AA10Z182);;国家高科技研究发展计划(863计划)资助项目(2007AA100105) |
| 摘 要： | 以中间锦鸡儿种子无菌萌发得茎段为外植体,不同激素组合诱导丛生芽,得出最佳丛生芽诱导培养基为MS+TDZ 0.1 mg/L+NAA 0.02 mg/L+糖30.0 g/L+琼脂9 g/L,芽诱导率达220%。诱导得到的丛生芽直接生根困难,需过渡培养。在培养基MS+BA 0.2 mg/L+NAA 0.4 mg/L+糖30.0 g/L+琼脂9 g/L上过渡培养三代以上后转入生根培养基1/2MS+IAA 0.5 mg/L+糖30.0 g/L+琼脂9 g/L生根,长出的根粗壮,且须根数多,生根率在85%以上。生根培养后在草炭土∶珍珠岩=4∶1的基质上移栽成活率达到80%。 |
| 关 键 词： | 中间锦鸡儿 TDZ 快繁体系 |
Inducing Regeneration of Caragana intermedia by TDZ |
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| HU Na-mei,HAN Su-ying,LIANG Guo-lu,QI Li-wan.Inducing Regeneration of Caragana intermedia by TDZ[J].Northern Horticulture,2009(8). | |
| Authors: | HU Na-mei HAN Su-ying LIANG Guo-lu QI Li-wan |
| Institution: | 1.College of Horticulture and Landscape;Southwest University;Chongqing 400716;China;2.Laboratory of Cell Biology;The Research Institute of Forestry;Chinese Academy of Forestry;Beijing 100091;China |
| Abstract: | An effective protocol for plant generation was developed from young stem segments of C.intermedia aseptic seeds.The results showed that the most suitable medium for inducing adventitious buds was MS+TDZ 0.1 mg/L+NAA 0.02 mg/L+sugar 30.0 g/L+agar 9 g/L,and the adventitious buds induction is up to 220% after 46 days cultivation.Regenerated buds grew strongly on MS+BA 0.2 mg/L+NAA 0.4 mg/L+sugar 30.0 g/L+agar 9 g/L.The rooting rate was 85% in the rooting medium 1/2MS+IAA 0.5 mg/L+sugar 30.0 g/L+agar 9 g/L afte... |
| Keywords: | Caraganaintrmedia TDZ Regeneration |
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