THE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) AS A SEROLOGICAL TEST FOR DETECTING ANTIBODIES AGAINST
LEPTOSPIRA INTERROGANS
SEROVAR
HARDJO
IN SHEEP |
| |
Authors: | B Adler S Faine L M Gordon† |
| |
Institution: | *Department of Microbiology, Monash University, Clayton, Victoria, 3168;‡Department of Agriculture, Regional Veterinary Laboratory, Hamilton, Victoria, 3300 |
| |
Abstract: | SUMMARY: The enzyme-linked immunosorbent assay (ELISA) was compared with the standard microscopic agglutination test (MAT) as a method for detecting antibodies against Leptospira interrogans serovar hardjo in sheep. Peak antibody levels detected by the 2 tests occurred at different times following experimental infection of sheep. In serums from flocks of sheep with naturally acquired infection there was a 95% correlation between MAT and ELISA with respect to the presence or absence of antibody to serovar hardjo , although the level of correlation of the titres of the 2 tests was low. The 2 tests appeared to measure different antigen-antibody systems. The ELISA would be a useful test for screening large numbers of serums for antibodies to L. interrogans serovar hardjo . |
| |
Keywords: | |
|
|