柿属植物叶绿体蛋白质编码基因密码子用法

为给后续柿属植物叶绿体基因工程研究提供基础,利用Codon W、CUSP、SPSS软件对5个柿属植物叶绿体基因组中的55条蛋白质编码基因序列进行密码子使用模式分析。结果显示,柿属植物叶绿体基因组密码子第3位碱基GC含量为26.9%~27.8%,有效密码子数在49.58~50.18之间,密码子偏好性较弱;柿叶绿体基因组不同位置的有效密码子数不同,取值范围为32.36~59.64;中性绘图分析和ENC-plot绘图分析结果表明,柿叶绿体基因组的密码子偏好性主要受选择的影响;通过高频密码子和高表达优越密码子分析相结合的方法,筛选出23个柿属植物最优密码子,且这些密码子主要以A和U碱基结尾。

Analysis on codon usage in the chloroplast protein-coding genes of Diospyros spp.

In order to provide basis for the follow-up studies on chloroplast genetic engineering in Diospyros spp.,codon usages of 55 chloroplast genes in five Diospyros spp species were analyzed by using CodonW,CUSP and SPSS sofiwares.The results showed that the GC content in the third codon position of the chloroplast genomes in Diospyros spp.was ranged from 26.9％ to 27.8％,and effective number of codons ranged firom 49.58 to 50.18,which indicated that the codon bias was weak.Effective number of codons at different location of the D.kakichloroplast genome was different,which ranged from 32.36 to 59.64.According to the results of neutrality plot analysis and ENC-plot analysis,codon bias in the chloroplast genome ofD.kakai was mainly affected by selection pressure.In addition,23 codons to be afftrmed as the optimal codons by using analysis of high-frequency codons and the high expression eodons,ending with A and U.