东乡野生稻原位圃遗传多样性的微卫星标记分析

遗传多样性的分析是收集与利用种质资源的重要前提。利用微卫星标记对113份东乡野生稻(Oryza rufipogon Griff.)(简称"东野")进行多样性分析,24对SSR引物共得到114个等位基因,平均每对引物得到4.75个,表明东野具有丰富的遗传多样性。聚类分析表明,东野群体内来自同一居群的部分材料遗传距离较近,3个居群中有2个居群最小遗传距离为0,1个居群最小遗传距离为0.018,但也有些材料间的遗传距离很大,最大达0.851。东野3个居群居群内的平均遗传距离为0.171~0.435,居群间的平均遗传距离为0.432~0.652,表明东野群体内的遗传变异以居群间为主,因此必须对东野原生地的现有居群实行有力的保护。

Genetic Diversity of In situ Conserved Dongxiang Wild Rice Detected by SSR

The genetic diversity analysis is important precondition for germ plasm resource's collection and utilization. The genetic diversity of 113 Dongxiang wild rice(Oryza rufipogon Griff.) was evaluated by 24 pairs of simple sequence repeat primers. A total of 114 alleles were generated with average allele per primer pair of 4.75, indicating that the Dongxiang wild rice had high genetic diversity. Clustering result showed that some individuals in the same colony were similar, the minimal genetic distances of the individuals in two colonies were 0 and in the other one was 0.018, the other individuals in the same colony were highly variant and the maximal genetic distance was 0.851. Genetic distance of intrinsic-populations were 0.171~0.435, being smaller than the values of inter-population which were 0.432~0.652. Consequently, the present populations of Dongxiang wild rice must be preserved strictly in original place.