紫叶酢浆草组培快繁技术研究 |
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作者姓名: | 孟月娥 周子发 李艳敏 赵秀山 |
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作者单位: | 河南省农科院园艺所,河南省农科院园艺所,河南省农科院园艺所,河南省农科院园艺所 郑州 450002,郑州 450002,郑州 450002,郑州 450002 |
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基金项目: | 河南省发展和改革委员会高新技术项目资助(0331990001). |
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摘 要: | 研究了紫叶酢浆草地下鳞茎初代培养、继代培养和生根培养3个技术环节的激素配比以及生 根苗移栽试验。结果表明:以MS为基本培养基,初代培养激素配比以6-BA0.5mg/L NAA0.5 mg/L 2,4-D0.1 mg/L为宜,诱导率达83%,增殖系数为2.6;在继代培养中,以6-BA0.5 mg/L NAA0.1 m/L 为宜,增殖系数达3以上;以1/2MS为基本培养基,生根激素以NAA0.1 mg/L为宜,可使生根率达 92%;生根苗移栽基质为蛭石与细沙按1:1时移栽成活率达90%。
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关 键 词: | 紫叶酢浆草 组培 快繁 激素配比 生根 移栽 |
Studies on Technique of Tissue Culture and Rapid Propagation of Oxalis triangularis purpurea |
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Authors: | Meng Yue'e Zhou Zif Li Yanmin Zhao Xiushan |
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Institution: | Institute of Horticulture, Henan Academy of A gricuture Science, Zhengzhou 450002 |
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Abstract: | Studies on hormone combinations of 3 kinds of culture including primary culture, subculture and rooting culture in the micropropagation method of Oxalis triangularis purpurea also on the transplanting conditions of rooted shoots. The experiments showed that the adventitious buds were induced to proliferation on MS medium supplemented with 6-BA 0.5 mg/L,NAA0.5 mg/L and 2,4-D 0.1 mg/L, the induction rate was 83%. On an average 2.6 shoots were formed per original explant in the initial culture. The number of shoots in subculture was more than 3 on MS medium supplemented with 6-BA 0.5 mg/L and NAA0.1 mg/L. The rooting rate of the shoots reached 92% on 1/2MS medium supplemented with 0.1 mg/L NAA; After transferred to a mixture with vermiculite and sands (l:l),about 90% of the rooted shoots were survival. |
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Keywords: | Oxalis triangularis purpurea Tissue culture Rapid propagation Hormone combination rooting Transplanting |
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