| 多溴联苯醚对大鼠肝细胞CYP1A2依赖性MROD活性的影响 |
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| 作者姓名： | 聂芳红 陈进军 Bunce Nigel |
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| 作者单位： | 广东海洋大学食品科技学院，广东海洋大学动物医学系，Dept．of Chemistry and Biochemistry University of Guelph，Guelph，Ontario，N1G 2W1，Canada，广东湛江 524088，广东湛江 524088 |
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| 基金项目： | 教育部留学回国人员科研启动基金（教外司留[20031406号）：广东海洋大学SCI源刊作者奖励基金（0412130）. |
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| 摘 要： | 采用未成熟SD大鼠肝细胞微粒体为试验材料,以2,3,7,8-四氯-二苯基-并-二哑噁英(2,3,7,8- tetrachlorobenzo-p-dioxin,TCDD)作为参照,利用甲氧基-异酚噁脱甲基酶(methoxyresorufin-O- demethylase,MROD)竞争性抑制动力学和荧光光谱法分析法,测定了5种多溴联苯(polybrominated diphenyl ethers,PBDEs)对7-甲氧基-异酚噁唑脱甲基反应的影响,间接研究了这些PBDEs对CYP1A2 的竞争性抑制作用。结果表明,和TCDD相比,PBDEs对MROD活性的抑制作用较弱,亦即对CYP1A2的 竞争性抑制作用较弱。
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| 关 键 词： | MROD分析法 CYP 1A2 多溴联苯醚 大鼠 |
MROD Analysis of Competitive Inhibition from Polybrominated Diphenyl Ethers to CYP 1A2 in Rat Hepatocytic Microsomes |
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| Authors: | Nie Fanghong Chen Jinjun Bunce Nigel |
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| Institution: | 1College of Food Science, Guangdong Ocean University, Zhanjiang, Guangdong 524088; 2Dept. of Veterinary Medicine, Guangdong Ocean University, Zhanjiang, Guangdong 524088; 3Dept. of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario, N1G 2W |
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| Abstract: | The role of a number of polybrominated diphenyl ethers (PBDEs) as inhibitors of the demethylation of 7 -methoxyresorufin, a process that is selectively catalyzed by cytochrome P450 1A2 (CYP1A2), was examined, using methoxyresorufin -O -demethylase (MROD) fluorescent assay and competitive inhibition constant Ki determination. It was found that those examined PBDEs showed punier inhibiting effects to MROD / CYP1A2 than that of 2,3,7,8-Tetrachlorobenzo-p-dioxin (TCDD) which is the strongest inhibitor of MROD / CYP1A2 activity in dioxin-like compounds. |
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| Keywords: | MROD assay CYP 1A2 Polybrominated diphenyl ethers Rat |
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