| 苏云金芽孢杆菌新菌株GGD-4杀虫晶体蛋白基因分析 |
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| 作者姓名： | 刘旭光 张广杰 郑霞 |
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| 作者单位： | 连云港师范高等专科学校初等教育系，连云港师范高等专科学校初等教育系，连云港师范高等专科学校自然科学系，连云港师范高等专科学校自然科学系 连云港222206连云港市应用生物技术重点实验室，连云港222206，连云港222206，连云港222206，连云港222206 |
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| 基金项目： | 连云港师范高等专科学校校级重点课题(LSZD200404)。 |
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| 摘 要： | 利用稀释平板培养法从连云港地区的土壤中分离了一株苏云金芽孢杆菌新菌株GGD-4,通过PCR-RFLP鉴定体系和SDS-PAGE方法分析了此菌株中cry基因类型和表达蛋白。结果表明:该菌株中含有cry1Aa基因型,同时表达130~140kD的蛋白;但EcoRⅠ和PstⅠ酶切结果不同于正常的cry1Aa基因,实验中利用生物信息学方法设计的cry1Aa基因特异引物对扩增后也证明含有cry1Aa基因。室内生测结果显示,该菌株对重要的农业害虫甜菜夜蛾、菜青虫等均有较高的杀虫毒力。
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| 关 键 词： | 苏云金芽孢杆菌 GGD-4 PCR-RFLP cry基因。 |
Analysis of cry-type Gene from Bacillus thuringiensis GGD-4 |
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| Authors: | Liu Xuguan Zhang Guangjie Zheng Xi Zhu Zhengyong |
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| Abstract: | In this study, cry-type genes from a new Bacillus thuringiensis GGD-4 stain, isolated by dilution-plate method from Lianyungang, had been identified using PCR-RFLP identification system, and SDS-PAGE analysis and bioassay had been performed. The results showed this strain containing cry1Aa type gene express 130~140kD proteins. However, the RFLP patterns of its cry1Aa profile were different from the others. With specific cry1Aa primers designed by bioinformation method, the evidence showed that this isolate also harbours cry1Aa gene by PCR. The bioassay in lab showed GGD-4 was high toxicity against two lepidoptera pests: Spodoptera exigua, Pl utella xylostella. |
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| Keywords: | Bacillus thuringiensis GGD-4 PCR-RFLP cry gene |
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