越南凉粉草无菌芽增殖生长的影响因子

【目的】探讨越南凉粉草无菌芽增殖生长的影响因子,为越南凉粉草离体快繁体系的建立提供技术参考。【方法】从培养基配方（不同基本培养基、不同蔗糖浓度及不同细胞分裂素CPPU）和外植体材料（不同接种部位）两方面研究其对越南凉粉草无菌芽增殖的影响。【结果】在MS、Miller与改良White3种基本培养基中,以MS对凉粉草芽增殖效果最好,芽增殖倍数达8.53倍;当蔗糖浓度为20.0g/L时芽增殖倍数最高,为8.34倍,而当蔗糖浓度为25.0g/L时芽增殖倍数为7.05倍,芽长势最好;添加CPPU对芽的增殖效果优于6-BA和KT,芽增殖倍数达9.80倍。对于外植体茎上部、茎中部和茎基部,以茎上部的芽增殖效果最好,芽增殖倍数达9.35倍,芽生长健壮。【结论】适宜越南凉粉草无菌芽增殖生长的基本培养基为MS培养基,蔗糖浓度为20.0～25.0g/L,CPPU为0.5mg/L,适宜接种材料为茎上部。

Study on effect factors for multiplication and growth of aseptic buds of Vietnam Mesona blumes

Objective The effect factors on multiplication and growth of the aseptic buds were studied in order to provide technical references for rapid propagation system in vitro of Vietnam Mesona blumes. Method The experiments were carried out from two aspects of media directions （different basic media, sucrose concentration and cytokinins） and explant materials （different inoculation positions）. ResultComparison of the basic media of MS, Miller and modified White displayed that MS medium showed the best effect on bud multiplication and growth of the aseptic buds, in which bud proliferation multiple was 8.53 times. While sucrose concentration was 20.0 g/L, bud proliferation multiple peaked at 8.34 times. When sucrose concentration reached 25.0 g/L, bud proliferation multiple showed 7.05 times and bud growth was the best. The bud multiplication effect of CPPU was better than that of 6-BA and KT, in which bud proliferation multiple reached 9.80 times. Comparing the inoculated materials from stem upper position, the middle part of the stem and the stem base position, the bud multiplication effect of stem upper position was the best, in which the bud proliferation multiple was 9.35 times and the bud growth exhibited the best status. Conclusion It was suitable to the bud multiplication and growth of Vietnam Mesona blumes that the basic medium was MS medium, 20.0-25.0 g/L sucrose concentration and 0.5 mg/L CPPU. The proper inoculated material was the stem upper position.