Isolation and characterization of lectins from the AG-D group of binucleate <Emphasis Type="Italic">Rhizoctonia</Emphasis> species |
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Authors: | Mwafaida J Mghalu Masashi Horibe Mayumi Kubota Hirokazu Kawagishi Mitsuro Hyakumachi |
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Institution: | (1) United Graduate School of Agricultural Sciences, Gifu University, Gifu, Japan;(2) Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, 501-1193 Gifu, Japan;(3) Faculty of Agriculture, Shizuoka University, Shizuoka, Japan |
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Abstract: | Isolates from 18 anastomosis groups (AGs) of binucleate Rhizoctonia were screened for lectin activity. Eight AGs (AG-B, AG-D, AG-F, AG-G, AG-H, AG-I, AG-R and AG-U) had low to moderate lectin
activities. Among these, members of AG-D and AG-I had the highest activity. Partially purified lectins from AG-D preferentially
agglutinated human blood type A to type B and O. Mucin and galactose were the most potent inhibitors among the tested carbohydrates.
The molecular masses of these lectins ranged from 12.7 kDa for the monomer to 62 kDa for the pentamer type. Proline, alanine,
glutamic acid, aspartic acid, leucine, threonine, serine and tyrosine were the major amino acid components of these lectins.
Lectins from AG-D were stable at 4–50°C and from pH 6.0 to 10.0. When assayed with isoelectric focusing, these lectins gave
bands at pI 9.30. Specificity of lectins from AG-D to galactose and its derivatives suggest a possible recognition role in
this fungal species. |
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Keywords: | Lectins Binucleate Rhizoctonia |
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