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4种胡椒科植物粗提物对灰葡萄孢的抑菌活性
引用本文:祁之秋,李修伟,纪明山. 4种胡椒科植物粗提物对灰葡萄孢的抑菌活性[J]. 中国蔬菜, 2018, 1(7): 64-67
作者姓名:祁之秋  李修伟  纪明山
作者单位:沈阳农业大学植物保护学院,辽宁沈阳 110161
基金项目:公益性行业(农业)科研专项(201303025)
摘    要:
在离体条件下比较了胡椒科荜茇、假蒟、黑胡椒、白胡椒4种植物乙醇提取物对灰葡萄孢的抑菌活性。结果表明:4种植物乙醇提取物对灰葡萄孢发育各阶段具有较强的抑制作用。白胡椒乙醇提取物对菌丝生长的抑制作用最强,EC_(50)为0.007 9 g·m L~(-1),其次为荜茇、黑胡椒和假蒟;当浓度为0.5 g·m L~(-1)时,荜茇和假蒟的乙醇提取物对灰葡萄孢产孢量抑制作用最强,抑制率分别为92.8%和92.0%;黑胡椒和白胡椒乙醇提取物对灰葡萄孢孢子萌发的抑制作用强于荜茇和假蒟;4种植物粗提物浓度高于0.062 5 g·m L~(-1)时,均能完全抑制灰葡萄孢菌核产生。

关 键 词:灰葡萄孢  胡椒科植物  粗提物  抑菌活性  

In Vitro Antifungal Activity of Four Pepper Plant Seeds Extracts Against Botrytis cinerea Pers
Qi Zhi-qiu,Li Xiu-wei,Ji Ming-shan. In Vitro Antifungal Activity of Four Pepper Plant Seeds Extracts Against Botrytis cinerea Pers[J]. China Vegetables, 2018, 1(7): 64-67
Authors:Qi Zhi-qiu  Li Xiu-wei  Ji Ming-shan
Affiliation:College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,Liaoning,China
Abstract:
In order to investigate the antifungal activity of pepper plant seeds extracts against Botrytis cinereaPers,the ethanol extract of pepper plant seeds was studied against all growth stages of Botrytis cinerea Pers in vitro.The results indicated that 4 pepper plant seeds had strong antifungal activity agaist Botrytis cinerea Pers. The whitepepper extract showed more inhibition on mycelium growth of Botrytis cinerea Pers than that of Piper longumL.,black pepper and Piper sarmentosum Roxb. with EC50 at 0.007 9 g·mL-1.The inhibition ratios of extractsof Piper longum L. and Piper sarmentosum Roxb. on spore product were 92.8% and 92.0% at 0.5 g·mL-1.Theantifungal activity of black pepper and white pepper extract on spore germination were higher than that of Piperlongum L. and Piper sarmentosum Roxb.Sclerotium product was inhibited,when the extract concentration of 4pepper plant seeds was higher than 0.062 5 g·mL-1
Keywords:Botrytis cinerea Pers  Pepper plant seeds  Extract  Antifungal activity  
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