BAD基因在敖汉细毛羊发情不同时期卵巢表达规律及对生殖激素的影响

本实验研究旨在探究BAD基因在敖汉细毛羊中的不同发情时期卵巢中表达量的变化规律以及对生殖激素的影响,为探讨其与绵羊发情的关系奠定基础。首先利用放射免疫方法测定敖汉细毛羊血液中促卵泡激素(FSH)、促黄体生成素(LH)、雌二醇(E_2)和孕激素(P_4)的浓度,然后利用qRT-PCR检测乏情期、发情前期、发情间期和发情期卵巢BAD基因的表达量变化。结果表明:卵巢中BAD基因在发情前期的表达量极显著高于乏情期、发情期和发情间期(P0.01),在发情期的表达量极显著高于乏情期和发情间期(P0.01),乏情期和发情间期的表达量基本相同(P0.05);E_2和LH浓度在BAD基因表达量最高的发情前期维持较高水平,P_4的浓度维持较低水平。综上可知,BAD基因的表达可能促进了E_2和LH浓度升高,抑制了P_4产生,进而影响细毛羊发情,可为绵羊发情的研究提供借鉴。

Expression Level of BAD Gene in Ovary of Aohan Fine-wool Sheep at Different Estrous Cycles

The aim of this study was to investigate the changes of expression of BAD gene in ovary under different estrus status in Aohan fine-wool sheep and the changes of reproductive hormone,this study explored the mechanism of BAD gene regulation in seasonal estrus control of Ao Han fine-wool sheep. This study chose the Ao Han fine-wool which is seasonal estrous sheepas the research model, First, the concentrations of FSH, LH, E2 and P4 in blood were measured by radioimmunoassay method. Then, its expression variations in ovary of anestrous stage, proestrus stage, peak estrous stage and dioestrus stage were detected by using qRT-PCR. The result showed that the expression levels of BAD gene were extremely high than anestrous, proestrus and dioestrus(P<0.01).The proestrus were extremely high than anestrous and dioestrus(P<0.01). The expression of anestrous and dioestrus is basically the same(P >0.05). The expression of BAD gene was highest in proestrus, meantime the concentration of E_2 and LH was at a high level, but the concentration of P_4 maintained in a relatively low level. It can be concluded that the expression of BAD gene may promote the increase of E_2 and LH, inhibit the secretion of P_4,and then regulate the oestrus of sheep.