| 基于高通量测序的不同填闲作物对黄瓜连作土壤微生物群落结构的影响 |
| |
| 引用本文： | 田晴,李敏,吴凤芝.基于高通量测序的不同填闲作物对黄瓜连作土壤微生物群落结构的影响[J].中国蔬菜,2018,1(10):60-68. |
| |
| 作者姓名： | 田晴 李敏 吴凤芝 |
| |
| 作者单位： | 东北农业大学园艺园林学院，黑龙江哈尔滨 150030 |
| |
| 基金项目： | 国家大宗蔬菜产业技术体系项目（CARS-23-B10） |
| |
| 摘 要： | 以连作3 a黄瓜的日光温室土壤为研究对象,以黄瓜为主栽作物,分别以大葱、普通白菜和小麦为冬夏季休闲期的填闲作物,采用高通量测序技术分析不同填闲模式对黄瓜连作土壤微生物群落结构的影响。结果表明:3种填闲模式处理的土壤细菌群落和真菌群落中的主要物种基本一致,但是相对丰度存在差异。在细菌属水平上,填闲小麦处理中Flexibacter、Nitrosomonadaceae_uncultured、Anaerolineaceae_unculture的相对丰度显著低于填闲大葱和普通白菜,而Flavobacterium、Clostridium、Peptostreptococcaceae_incertae_sedis的相对丰度显著高于填闲大葱和普通白菜;在真菌属水平上,填闲小麦处理中Ascomycota_unclassified、Hypocreales_unclassified、Humicola的相对丰度显著低于填闲大葱和普通白菜,而Fusarium、Pyrenochaeta、Nectriaceae_unclassified、Preussia的相对丰度显著高于填闲大葱和普通白菜。说明不同填闲作物对土壤微生物群落结构和多样性的影响存在差异。
|
| 关 键 词： | 黄瓜 连作土壤 填闲作物 高通量测序 真菌群落 细菌群落 |
Effects of Different Cover Crops on Soil Microbial Community Structure in Cucumber Continuous Cropping Based on High-throughput Sequencing |
| |
| TIAN Qing,LI Min,WU Feng-zhi.Effects of Different Cover Crops on Soil Microbial Community Structure in Cucumber Continuous Cropping Based on High-throughput Sequencing[J].China Vegetables,2018,1(10):60-68. |
| |
| Authors: | TIAN Qing LI Min WU Feng-zhi |
| |
| Institution: | College of Horticulture and Landscape Architecture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China |
| |
| Abstract: | Taking the solar greenhouse soil for 3 years successive cucumber cropping as research object，
this paper analyzed the effects of different filling patterns on soil microbial community structure by high-throughput
sequencing technology under successive cucumber cropping．In this study，cucumber was the main crop，
scallion，cole and wheat were the crops for winter and summer leisure period．The results indicated that the
major species in soil bacterial community and fungus community by 3 different filling patterns basically had no
difference，but there were differences in relative abundances among them．At the level of bacteria，among filling
wheat treatment，the relative abundances of Flexibacter，Nitrosomonadaceae_uncultured，naerolineaceae_
unculture were significantly lower than those of scallion and cole．While，the relative abundances of
Flavobacterium，Clostridium and Peptostreptococcaceae_incertae_sedis were significantly higher than those of
scallion and cole．At the level of fungus，among filling wheat treatment，the relative abundances of Ascomycota_
unclassified，Hypocreales_unclassified，Humicola were significantly lower than those of scallion and cole．
While，the relative abundances of Fusarium，Pyrenochaeta，Nectriaceae_unclassified and Preussi were
significantly higher than those of scallion and cole．These results indicated that there were differences in the effects
of different cover crops on soil microbial community structure and diversity． |
| |
| Keywords: | Cucumber Continuous cropping Cover crops High-throughput sequencing Fungal community Bacterial community |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国蔬菜》浏览原始摘要信息 |
| 点击此处可从《中国蔬菜》下载免费的PDF全文 |
