土耳其斯坦叶螨对阿维菌素和哒螨灵抗药性机理及抗性适合度研究

目的]明确土耳其斯坦叶螨对阿维菌素和哒螨灵的抗药性机理和抗性适合度.方法]在28℃条件下对土耳其斯坦叶螨敏感品系进行室内抗药性选育,选育出对阿维菌素和哒螨灵的抗性品系,组建生命表进行抗性适合度研究.并比较抗性品系和敏感品系的几种常见解毒酶的活性测定来进行抗性机理研究.结果]土耳其斯坦叶螨对阿维菌素的抗性发展比较慢.在抗性适合度的研究中发现土耳其斯坦叶螨的两种抗性品系在发育历期、产卵量等指标跟敏感品系相比均存在不利性.并发现羟酸酯酶在两种抗性品系和敏感品系中比活力表现不明显,在抗阿维菌品系和敏感系中碱性磷酸酯酶存在差异性,这说明这种酶在解毒阿维菌素过程中起作用.谷胱甘肽S-转移酶、多功能氧化酶、酸性磷酸酯酶和碱性磷酸酯酶在抗哒螨灵品系和敏感品系中均表现差异.说明这些酶在解毒哒螨灵的过程中起作用.结论]阿维菌素和哒螨灵的抗性与解毒酶活性的变化有关,并且在产生抗性后存在适合度不利性.

Study on Pesticide Resistance Mechanisms and Resistant Fitness of Tetranychus turkestani in Abamectin and Pyridaben

【Objective】To study pesticide resistance mechanisms and resistant fitness of Tetranychus turkestan to Abamectin and Pyridaben.【Method】The resistant strains of T.turkestani to Abamectin and Pyridaben through indoor's breeding of the susceptible strain were selected under 28℃ conditions.And resistant fitness of T.turkestani by constructing the life table were studied.Research on pesticide resistance mechanisms were finished by the determination on several common detoxifying enzymes activity.【Result】Development on the resistance to Abamectin was slow in the process of selecting resistant strains.Compared with the susceptible strain,both the two resistant strains have adverse effect in development duration,egg production and some other indicators,which is suggested in the study of resistant fitness.The research on pesticide resistance mechanisms shows that it is unconspicuous differernt between the three kinds of Tetranychus turkestan on CarE.There are different between S and PyR on the PLA,so PLA have effect on it.The effect of ACP、ALP、MFO and GST are all different between S and AbR.It showed that these enzymes are all detoxifcation of the Pyridaben.【Conclusion】This research shows the resistance of Abamectin and Pyridaben were related to activity of detoxification enzymes.There are adverse effect on fitness of T.turkestan after the occurrence of resistance.