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| 辣椒CMV广州分离物的CP基因序列分析及亚组鉴定 | |
| 引用本文： | 孙秀东,周淑梅,雷建军,曹必好,陈国菊.辣椒CMV广州分离物的CP基因序列分析及亚组鉴定[J].山东农业科学,2008(9). |
| 作者姓名： | 孙秀东 周淑梅 雷建军 曹必好 陈国菊 |
| 作者单位： | 1. 山东农业大学园艺科学与工程学院,山东,泰安,271018 2. 山东农业大学生命科学学院,山东,泰安,271018 3. 华南农业大学园艺学院,广东,广州,510642 |
| 基金项目： | 广东省攻关项目 ， 广州市攻关项目 ， 广东省农业厅项目资助 |
| 摘 要： | 从广州市呈现花叶病症的辣椒上分离纯化得到1个黄瓜花叶病毒的分离物(CMV-GZ)。利用RT-PCR技术克隆了该病毒分离物的外壳蛋白(CP)基因,其序列长度为657 bp,编码218个氨基酸。对CP基因序列进行同源性比较分析,结果表明该病毒分离物的CP基因与CMV亚组Ⅰ5个株系的同源性均在90%以上,而与亚组Ⅱ4个株系的同源性均低于80%。据此将该分离物归属于CMV亚组Ⅰ。 |
| 关 键 词： | 辣椒 CMV 亚组Ⅰ CP基因 序列分析 |
Sequence Analysis and Subgroup Identification of CP Gene in A CMV Guangzhou Isolate from Pepper (Capsicum annuum) |
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| SUN Xiu-dong,ZHOU Shu-mei,LEI Jian-jun,CAO Bi-hao,CHEN Guo-ju.Sequence Analysis and Subgroup Identification of CP Gene in A CMV Guangzhou Isolate from Pepper (Capsicum annuum)[J].Shandong Agricultural Sciences,2008(9). | |
| Authors: | SUN Xiu-dong ZHOU Shu-mei LEI Jian-jun CAO Bi-hao CHEN Guo-ju |
| Institution: | SUN Xiu-dong1,ZHOU Shu-mei2,LEI Jian-jun3,CAO Bi-hao3,CHEN Guo-ju3 |
| Abstract: | A CMV isolate(CMV-GZ) was obtained from the pepper with mosaic symptom in Guangzhou.The coat protein(CP) gene of the isolate was cloned by RT-PCR.Sequencing results showed that the CP gene of the isolate was 657 bp,encoding 218 amino acids.The homology analysis indicated that the CP gene nucleotide sequences of the isolate shared over 90% homology with 5 strains of CMV subgroupⅠ,but less than 80% with 4 strains of subgroupⅡ.So it was concluded that the isolate CMV-GZ belonged to subgroupⅠ. |
| Keywords: | CMV |
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