| 马缨杜鹃下胚轴组织培养及全息现象的研究 |
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| 引用本文： | 周艳,高贵龙,邹天才,黄丽华,周洪英,陈训. 马缨杜鹃下胚轴组织培养及全息现象的研究[J]. 种子, 2010, 29(1) |
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| 作者姓名： | 周艳 高贵龙 邹天才 黄丽华 周洪英 陈训 |
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| 作者单位： | 1. 贵州省植物园,贵阳,550001
2. 贵州科学院,贵阳,550001 |
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| 基金项目： | 贵州省科技得大专项，国家农业成果转化课题资助项目 |
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| 摘 要： | 以马缨杜鹃下胚轴为外植体,用L9(34)正交实验法研究基本培养基、NAA、2-ip对芽器官诱导的影响。以马缨杜鹃下胚轴切断为材料,研究全息现象。结果表明马缨杜鹃下胚轴诱导芽的最佳培养配方为1/4 MS+NAA0.75 mg/L+2-ip2.0 mg/L。马缨杜鹃切段在离体培养时存在全息现象,其不定芽发生的部位及数量、频率的梯度变化都遵循生物全息律,全息律的呈现在一定程度上受外源激素和切断位置的影响。
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| 关 键 词： | 马缨杜鹃 组织培养 下胚轴 全息现象 |
Research on the Tissue Culture and the Holographic Phenomenon of Rhododendron delavayi Franch.Hypocotyl |
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| ZHOU Yan,GAO Gui-long,ZOU Tian-cai,HUANG Li-hua,ZHOU Hong-ying,CHEN Xun. Research on the Tissue Culture and the Holographic Phenomenon of Rhododendron delavayi Franch.Hypocotyl[J]. Seed, 2010, 29(1) |
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| Authors: | ZHOU Yan GAO Gui-long ZOU Tian-cai HUANG Li-hua ZHOU Hong-ying CHEN Xun |
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| Affiliation: | ZHOU Yan1,GAO Gui-long2,ZOU Tian-cai1,HUANG Li-hua2,ZHOU Hong-ying1,CHEN Xun2(1.Guizhou Botanical Garden,Guiyang 550001,China,2.Guizhou Academy of Sciences Guiyang,China) |
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| Abstract: | Using Rhododendron delavayi Franch hypocotyl as explant,through the L_9(3~4) orthogonal experiment,a study was conducted to study the effect of tissue culture on organs sprouting by basic culture medium、NAA、2-ip.Moreover,researched bioholographic phenomenon by hypocotyl cutting tissue culture.Results showed that the optimal combination of bud differentiation Was 1/4 MS+0.75 ms/L NAA+2-ip 2.0 mg/L.It Was found that in vitro culture of hypocotyl segments showed holographic phenomenon;the position and the quantity、the frequency gradient of sprouting inducing all conformed to the bioholographic law,the hormone and the position of hypocotyl could effect the bioholographic law to a certain extent. |
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| Keywords: | Rhododendron delavayi Franch. plant tissue culture hypocotyl bioholographic phenomenon |
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