球孢白僵菌诱导酶发酵液对其毒力的增效作用

为进一步确认含蛋白酶、几丁质酶、脂肪酶和淀粉酶的诱导酶发酵液对球孢白僵菌(Beauveria bassiana)菌株毒力的影响,将高毒力菌株B5、Bxs与低毒力菌株B11、BZ分别制成1×107个/mL孢悬液(I型)、孢悬液+诱导酶发酵液(V∶V=1∶1;II型)、孢悬液+灭菌诱导酶发酵液(V∶V=1∶1;III型),用浸渍法分别测定I型、II型与III型3种液体对松墨天牛(Monochamus alternatus)幼虫的毒力。结果表明:分别含有蛋白酶、几丁质酶和脂肪酶的诱导酶发酵液Ⅱ型液体,其LT50值与Ⅲ型液体LT50间的差异均为极显著(P0.01),而含淀粉酶的差异不显著。分别含有4种酶的诱导酶发酵液的Ⅲ型与Ⅰ型液体相比,LT50值之间也存在极显著差异(P0.01)。可见:含蛋白酶、几丁质酶和脂肪酶的诱导酶发酵液对球孢白僵菌毒力具有极显著的增效作用;经过酶灭活的4种诱导酶发酵液增效作用也极显著。

Synergistic effect of induced enzyme fermented solution on the insecticidal efficacy of Beauveria bassiana

In order to evaluate the toxicity effect of induced enzyme fermented solution of protease, chitinase, lipase and amylase from Beauveria bassiana, 3 types of solutions were prepared under laboratory conditions, i.e.,1×107/mLspore suspension (type Ⅰ), spore suspension + induced enzyme fermented solution (V:V=1: 1, type Ⅱ) and spore suspension + sterilized induced enzyme fermented solution (V:V=1:1, type Ⅲ), and microbial virulence against Monochamus alternatus larvae were tested by immersion method. The results showed that the LT50 values of induced protease, chitinase and lipase fermented solution(type Ⅱ)were significantly different from that of type Ⅲ solution (P50 values was not significantly different between the two types of solution. Moreover, there were significant difference between type Ⅲ and type Ⅰ fermented solution of 4 kinds of enzyme (PB. bassiana, and the inactivated 4 kinds of fermented solution also showed significant synergistic effect.