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奶牛场常用乳头药浴剂抑菌效果观察
引用本文:罗丹丹,王文贵,于吉峰,叶勇刚,廖党金,汪明,谢晶,李江凌.奶牛场常用乳头药浴剂抑菌效果观察[J].中国奶牛,2012(3):25-28.
作者姓名:罗丹丹  王文贵  于吉峰  叶勇刚  廖党金  汪明  谢晶  李江凌
作者单位:1. 四川省畜牧科学研究院,成都,610066
2. 中国农业大学,北京,100193
基金项目:国家支撑计划项目(2006BAD04A17、2008BAK51B08);国家奶牛产业技术体系科学家岗位、四川省支撑计划项目(2010NZ0106)
摘    要:采用平板细菌计数法对四个牛场奶牛乳头药浴前、后的细菌总数进行统计分析。结果显示A、B、C三个牛场乳头药浴前与药浴后的细菌总数差异极显著(P<0.05),D牛场乳头药浴前与药浴后的细菌总数无统计学差异(P>0.05)。表明A~C场奶牛乳头药浴剂效果较好,D场效果欠佳。此外,对培养的细菌进行革兰氏染色和镜检,结果显示主要有沙门氏菌、大肠杆菌、葡萄球菌和枯草芽孢杆菌,与文献报道相符。

关 键 词:细菌计数  差异显著性检验  革兰氏染色

Observation on the Bacteriostatic Effect of Cow's Medicated Balneum
LUO Dan-dan,WANG Wen-gui,YU Ji-feng,YE Yong-gang,LIAO Dang-jin,WANG Ming,XIE Jing,LI Jiang-ling.Observation on the Bacteriostatic Effect of Cow's Medicated Balneum[J].China Dairy Cattle,2012(3):25-28.
Authors:LUO Dan-dan  WANG Wen-gui  YU Ji-feng  YE Yong-gang  LIAO Dang-jin  WANG Ming  XIE Jing  LI Jiang-ling
Institution:1. SichuanAcademy of Animal Science, Chengdu 610066; 2. China Agricultural University, Beijing 100193)
Abstract:The significance test of difference of the total bacteria medicated bath before and after, which was calculated by the method of Plate bacteria counts, was researched in this paper. The results showed that the total bacteria medicated bath before and after was extremely significant for A, B and C dairy farms(P〈0.05), but not significant for D dairy farm (P〉0.05). It is concluded that the medicated balneum of A, B and C dairy farms was better than that of D dairy farm. In addition, salmonella, bacillus coli, staphylococci and bacillus were detected by gram staining and microscopic examination.
Keywords:Bacteria counts  Significance test of difference  Gram staining
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