| 甘蓝和油菜中类硫氧还蛋白THL2基因的克隆与序列分析 |
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| 引用本文： | 王茂广,吴俊岩,吴能表,朱利泉,王小佳.甘蓝和油菜中类硫氧还蛋白THL2基因的克隆与序列分析[J].园艺学报,2004,31(3):381-383. |
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| 作者姓名： | 王茂广 吴俊岩 吴能表 朱利泉 王小佳 |
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| 作者单位： | (西南农业大学农学与生命科学学院生化实验室，重庆400716； 西南农业大学园艺园林学院，重庆市蔬菜学重点实验室，重庆400716) |
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| 基金项目： | 国家自然科学基金资助项目 ( 3 0 170 64 6)，重庆市应用基础项目 ( 6870 ) |
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| 摘 要： | 采用PCR和RT-PCR方法，对甘蓝和油菜的基因组DNA和柱头总RNA进行扩增，获得了类硫氧还蛋白THL2的DNA和cDNA。序列分析首次表明THL2基因有两个内含子，并且在油菜和甘蓝中的大小不同。进一步分析表明，油菜和甘蓝中THL2 DNA序列的同源性为96％。
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| 关 键 词： | 自交不亲和性 THL2基因 克隆 序列分析 |
| 文章编号： | 0513-353X(2004)03-0381-03 |
| 收稿时间： | 2003-07-15 |
Cloning and Sequence Analysis of Thioredoxin-h-like THL2 Gene in Brassica napus L.And Brassica olereacea L. |
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| Wang Maoguang ,Wu Junyan ,Wu Nengbiao ,Zhu Liquan ,and Wang Xiaojia.Cloning and Sequence Analysis of Thioredoxin-h-like THL2 Gene in Brassica napus L.And Brassica olereacea L.[J].Acta Horticulturae Sinica,2004,31(3):381-383. |
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| Authors: | Wang Maoguang Wu Junyan Wu Nengbiao Zhu Liquan and Wang Xiaojia |
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| Institution: | (Laboratory ofBiochemistry，College ofAgronomy and Life Sciences，Southwest Agricultural University，Chongqing 400716，China； Chongqing Key Laboratory of Olericulture，College of Horticulture and Gardens，Southwest Agricultural University，Chongqing 400716．China) |
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| Abstract: | Thioredoxin h like protein THL 2 DNA and cDNA were amplified from genomic DNA and stigma total RNA in Brassica napus L. and B olereacea L by PCR and RT PCR method respectively. Sequence analysis first indicated that THL 2 gene contained two introns,which were different in size between B napus L and B olereacea L Homologous analysis demonstrated that there was 96% identity of THL 2 DNA from B.napus L and B olereacea L.. |
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| Keywords: | Self incompatibility THL 2 gene Cloning Sequence analysis |
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