| 不同激素配比对红花石蒜小鳞茎及茎尖的分化培养的影响 |
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| 引用本文： | 林田,刘灶长,李天菲,郑海柔,罗利军.不同激素配比对红花石蒜小鳞茎及茎尖的分化培养的影响[J].上海农业学报,2006,22(4):45-47. |
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| 作者姓名： | 林田 刘灶长 李天菲 郑海柔 罗利军 |
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| 作者单位： | 1. 上海市农业生物基因中心,上海,201106;华中农业大学,武汉,430070
2. 上海市农业生物基因中心,上海,201106 |
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| 基金项目： | 上海市科技兴农攻关项目[沪农科攻字(2005)第1-7号]资助 |
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| 摘 要： | 以红花石蒜(Lycoris radiata)的鳞茎切块及茎尖为材料,采用不同激素配比的MS培养基,对离体小鳞茎的分化和增殖进行了研究。结果表明,BA 3 mg/L及NAA 1.5 mg/L配比获得的小鳞茎诱导频率最高,可达53%,诱导生成的小植株强健整齐。鳞茎茎尖培养可形成多种不同类型的愈伤组织,本试验中有2种类型愈伤组织转入分化培养基后可产生大量的小鳞茎。
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| 关 键 词： | 石蒜 组织培养 小鳞茎 茎尖培养 愈伤组织 |
| 文章编号： | 1000-3924（2006）04-45-03 |
| 收稿时间： | 07 6 2006 12:00AM |
| 修稿时间： | 2006-07-062006-11-09 |
Effects of different phytohormone combinations on bulblet induction and bulb-tip regeneration of Lycoris radiata |
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| LIN Tian,LIU Zao-chang,LI Tian-fei,ZHENG Hai-rou,LUO Li-jun.Effects of different phytohormone combinations on bulblet induction and bulb-tip regeneration of Lycoris radiata[J].Acta Agriculturae Shanghai,2006,22(4):45-47. |
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| Authors: | LIN Tian LIU Zao-chang LI Tian-fei ZHENG Hai-rou LUO Li-jun |
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| Institution: | 1 Shanghai Agro-biological Gene Center, Shanghai 201106, China ; 2 Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | Tissue culture was carried out to study the effect of different phytohormone combinations in MS medium on the bulblet induction and micro propagation from scale cuttings and bulb tip explants of Lycoris radiate.The result showed that the MS medium with the phytohormones of 3mg/L BA 1.5mg/L NAA was the best for differentiation of vigorous and consistent bulblets and the inductivity reached 53%.Several types of calli had been induced from bulb tips and two of them differentiated into many bulbltes. |
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| Keywords: | Lycoris radiata Tissue culture Bulblet Bulb-tip culture Callus |
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