| 猪脑心肌炎病毒抗体间接ELISA检测方法的建立及应用 |
| |
| 引用本文： | 陆文俊,施开创,屈素洁,莫胜兰,李向涛,钟诚.猪脑心肌炎病毒抗体间接ELISA检测方法的建立及应用[J].动物医学进展,2011(12):6-10. |
| |
| 作者姓名： | 陆文俊 施开创 屈素洁 莫胜兰 李向涛 钟诚 |
| |
| 作者单位： | 广西动物疫病预防控制中心;广西大学动物科学技术学院 |
| |
| 基金项目： | 广西科学基金项目(桂科青0728047) |
| |
| 摘 要： | 以猪脑心肌炎病毒(Encephalomyocarditis virus,EMCV)VP1重组蛋白为包被抗原,建立了检测EMCV血清抗体的间接酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA).经优化,获得间接ELISA的最佳反应条件为抗原包被浓度0.625 μg/mL,待...
|
| 关 键 词： | 猪 脑心肌炎病毒 VP1重组蛋白 间接ELISA 血清学调查 |
Development and Application of an Indirect ELISA for Detection of Antibody Against Porcine Encephalomyocarditis Virus |
| |
| LU Wen-jun,SHI Kai-chuang,QU Su-jie,MO Sheng-lan,LI Xiang-tao,ZHONG Cheng.Development and Application of an Indirect ELISA for Detection of Antibody Against Porcine Encephalomyocarditis Virus[J].Progress In Veterinary Medicine,2011(12):6-10. |
| |
| Authors: | LU Wen-jun SHI Kai-chuang QU Su-jie MO Sheng-lan LI Xiang-tao ZHONG Cheng |
| |
| Institution: | 1.Guangxi Center for Animal Disease Control and Prevention,Nanning,Guangxi,530001,China;2.College of Animal Science and Technology,Guangxi University,Nanning,Guangxi,530005,China) |
| |
| Abstract: | An indirect ELISA for detection of antibody against porcine encephalomyocarditis virus(EMCV) was developed with the EMCV recombinant protein VP1 as antigen.After optimization,the optimal reaction conditions of the indirect ELISA were determined.The results showed that the optimal concentration of the coated antigen was 0.625 μg/mL;the dilution of serum samples was 1∶40;the optimal blocking solution was 50 g/L skim milk;the working concentration of HRP-labeled rabbit anti-porcine IgG was 1∶6 000;the coloration time was 15 min at room temperature;the threshold value of positive and negative serum samples was 0.33 at OD450nm.The recombinant antigen had no cross reaction with antibodies against FMDV,CSFV,PRRSV,PRV and PCV-2,respectively.The established indirect ELISA was used to detect EMCV antibody in 2 228 serum samples collected from Guangxi in 2010-2011.The results showed that the positive rate of serum samples was 91.47%,and 100% pig farms existed positive serum samples,indicating that EMCV infection was very common in pig farms of Guangxi region. |
| |
| Keywords: | porcine Encephalomyocarditis virus(EMCV) recombinant protein VP1 indirect ELISA serological survey |
| 本文献已被 CNKI 等数据库收录！ |
|
