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“恩钿”月季组培技术研究
引用本文:龚维红,尤伟忠,李成慧,葛彩燕.“恩钿”月季组培技术研究[J].北方园艺,2012(16):83-85.
作者姓名:龚维红  尤伟忠  李成慧  葛彩燕
作者单位:苏州农业职业技术学院,江苏苏州,215008;苏州农业职业技术学院,江苏苏州,215008;苏州农业职业技术学院,江苏苏州,215008;苏州农业职业技术学院,江苏苏州,215008
基金项目:江苏省高等学校大学生实践创新训练计划资助项目,苏州农业职业技术学院院级资助项目
摘    要:以"恩钿"月季茎段上不同部位、不同季节的芽为外植体,研究了"恩钿"月季组培苗的最佳培养基。结果表明:外植体选取时间以春季为佳,选取部位以枝条中、上部为好,适宜的增殖培养基为:MS+6-BA 1.0mg/L+NAA 0.1mg/L,最佳生根培养基为:1/2MS+NAA 0.2mg/L+300mg/L活性炭;练苗基质以蛭石为佳。

关 键 词:“恩钿”月季  组织培养  培养基

Study on Tissue Culture Technique of China Rose of 'Entian'
GONG Wei-hong , YOU Wei-zhong , LI Cheng-hui , GE Cai-yan.Study on Tissue Culture Technique of China Rose of 'Entian'[J].Northern Horticulture,2012(16):83-85.
Authors:GONG Wei-hong  YOU Wei-zhong  LI Cheng-hui  GE Cai-yan
Institution:(Suzhou Polytechnical Institute of Agriculture,Suzhou,Jiangsu 215008)
Abstract:Taking the bud of stem-segment in different seasons and different positions as the explants,the tissue culture technique was studied.The results showed that the spring was the best time for selection of explants;the medium and top portion of trees were the right position for the explants cut.The proliferation media formula was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L;the media formula for rooting was 1/2MS+NAA 0.2 mg/L+300 mg/L active carbon.Vermiculite was taken as the media for domestication.
Keywords:China rose of ’Entian’  tissue culture  media
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