水稻中胚轴长度QTL分析

为分析水稻中胚轴伸长与赤霉素的关系及其遗传基础,以沈农265(长中胚轴)和丽江新团黑谷(短中胚轴)的RIL群体为材料,结合其连锁图谱,对水和赤霉素溶液两种培养条件下的中胚轴长度进行QTL定位。结果表明,浓度为1.50μmolL~(-1)的赤霉素可显著促进中胚轴的伸长。两种培养条件下,共检测到控制中胚轴长度的5个QTL,分布在第1、第2、第3、第6和第11号染色体上,LOD值在3.65～15.52范围内,单个QTL对表型贡献率在7%～33%之间。其中qML3、qML6和qML11在2种处理条件下均被检测到,qML1和qML2仅在水培条件下被检测到。与其他研究比较发现,主效基因qML3可以在不同群体和不同环境下稳定表达。

QTL Analysis for Mesocotyl Length in Rice(Oryza sativa L.)

To understand genetic basis underlying the relationship between mesocotyl elongation and hormone (GA) in rice (Oryza sativa L.), we genetically analyzed RILs population derived from a cross between two japonica rice cultivars, Shennong 265 (long mesocotyl) and Lijiangxintuanheigu (short mesocotyl), with its linkage map. QTLs for mesocotyl length were detected under water and GA germination conditions. The results showed that GA at 1.50 mmol L^-1 stimulated mesocotyl elongation dramatically. Total five QTLs for rice mesocotyl length, each accounting for 7% to 33% of the phenotypic variance (VE), were detected on chromosomes 1, 2, 3, 6, and 11 under two germination conditions (environments). The LOD value of each QTL was ranged from 3.65 to 15.52. Among the five QTLs, qML3, qML6, and qML11 were detected under both germination conditions and qml1 and qml2 were identified only under water germination condition. In comparison with other studies revealed that major QTL qML3 was easily re-detected in different populations and environments.