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Isolation of a pure thornless loganberry by meristem tip culture
Authors:H K Hall  M H Quazi  R M Skirvin
Institution:(1) Crop Research Division, DSIR, Private Bag, Christchurch, New Zealand;(2) Present address: Riwaka Research Station, DSIR, RD 3, Motueka, New Zealand;(3) Present address: Department of Plant Breeding and Genetics, N.W.F.P. Agricultural University, Peshawar, Pakistan;(4) Present address: Department of Horticulure, University of Illinois, 1707 So. Orchard, 61801 Urbana, Illinois, USA
Abstract:Summary lsquoThornless Loganberryrsquo (TL) is a periclinal chimeral blackberry in which a layer of mutant (thornless) epidermis surrounds a core of wild-type (thorny) tiusse. Due to its chimeral arrangement, TL produces thorny adventitious root cuttings and thorny offspring. To separate the chimera into its components parts, meristems of TL were grow in vitro on modified Murashige and Skoog medium to yield callus and adventitious shoots. One of these shoots has survived, flowered, and produced thornless offspring from seed. The importance of this non-chimeral TL is discussed.List of terms BA 6-benzylaminopurine - GA3 gibberellic acid - NAA naphthalene acetic acid - hybridberry polyploid bramble interspecific hybrids - MS Murashige & Skoog (1962) high mineral salt medium - TL lsquoThornless Loganberryrsquo - TLtc tissue culture-derived (non-chimeral) lsquoThornless Loganberryrsquo
Keywords:Rubus  blackberry  raspberry  hybridberry  Loganberry  chimera  in vitro  plant breeding  tissue culture  thornless
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