Isolation of a pure thornless loganberry by meristem tip culture |
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Authors: | H K Hall M H Quazi R M Skirvin |
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Institution: | (1) Crop Research Division, DSIR, Private Bag, Christchurch, New Zealand;(2) Present address: Riwaka Research Station, DSIR, RD 3, Motueka, New Zealand;(3) Present address: Department of Plant Breeding and Genetics, N.W.F.P. Agricultural University, Peshawar, Pakistan;(4) Present address: Department of Horticulure, University of Illinois, 1707 So. Orchard, 61801 Urbana, Illinois, USA |
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Abstract: | Summary Thornless Loganberry (TL) is a periclinal chimeral blackberry in which a layer of mutant (thornless) epidermis surrounds a core of wild-type (thorny) tiusse. Due to its chimeral arrangement, TL produces thorny adventitious root cuttings and thorny offspring. To separate the chimera into its components parts, meristems of TL were grow in vitro on modified Murashige and Skoog medium to yield callus and adventitious shoots. One of these shoots has survived, flowered, and produced thornless offspring from seed. The importance of this non-chimeral TL is discussed.List of terms BA
6-benzylaminopurine
- GA3
gibberellic acid
- NAA
naphthalene acetic acid
- hybridberry
polyploid bramble interspecific hybrids
- MS
Murashige & Skoog (1962) high mineral salt medium
- TL
Thornless Loganberry
- TLtc
tissue culture-derived (non-chimeral) Thornless Loganberry |
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Keywords: | Rubus blackberry raspberry hybridberry Loganberry chimera in vitro plant breeding tissue culture thornless |
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