Molecular characterization and variability analysis of <Emphasis Type="Italic">Apple scar skin viroid</Emphasis> in India |
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| Authors: | Yashika Walia Yogesh Kumar Tanuja Rana Pooja Bhardwaj Raja Ram Pritam Das Thakur Usha Sharma Vipin Hallan A A Zaidi |
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| Institution: | (1) Plant Virology Lab, Institute of Himalayan Bioresource Technology, CSIR, Palampur, Himachal Pradesh, 176061, India;(2) Department of Mycology and Plant Pathology, Dr. Y. S Parmar University, Nauni, Solan, Himachal Pradesh, India;(3) KVK, Rohru, Shimla, Himachal Pradesh, India |
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| Abstract: | Apple scar skin viroid (ASSVd) infection is a major limitation to apple fruit quality and causes huge economic losses. In surveys of apple orchards
in the northern Indian state of Himachal Pradesh, fruits with dappling symptoms were noticed. ASSVd was detected from these
fruits and molecularly characterized. Ten clones from three isolates were sequenced, of which seven were new sequence variants
of ASSVd. The clones had significant sequence variability (94–100%) with each other. Variability was more common in the pathogenic
domain of the viroid genome. Four of the clones were 330 nucleotides (nt) long, and the other six had an additional nucleotide.
Phylogenetic analysis showed close affinity of the present isolates with some Chinese and Korean isolates. The study reports
seven new variants of ASSVd and also provides the first molecular evidence of viroid infection (ASSVd) in apple in India. |
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| Keywords: | Apple scar skin viroid Cloning DNA sequencing Phylogenetic analysis |
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