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| 三氯化铬对大鼠胰岛细胞活性及胰岛素分泌的影响 | |
| 引用本文： | 张宏馨,李兰会,杨宏莉,王燕,李军,廉海晨,卢淑兰.三氯化铬对大鼠胰岛细胞活性及胰岛素分泌的影响[J].河北农业大学学报,2009,32(5). |
| 作者姓名： | 张宏馨 李兰会 杨宏莉 王燕 李军 廉海晨 卢淑兰 |
| 作者单位： | 1. 河北大学,医学部,河北,保定,071001 2. 河北农业大学,动物科技学院,河北,保定,071001 3. 河北省荣军医院,河北,保定,071001 |
| 基金项目： | 河北省卫生厅项目，河北省科技计划项目，河北大学医学部科学研究基金项目 |
| 摘 要： | 为了研究三氯化铬与大鼠胰岛细胞共培养对胰岛细胞活性与胰岛素分泌水平的影响,并对其影响机制进行探讨,以台盼蓝染色法和MTT法检测胰岛β细胞活性,以不同浓度葡萄糖刺激胰岛素释放评价三氯化铬对胰岛细胞功能的影响,同时以RT-PCR检测胰岛细胞的抗凋亡基因bcl-2的表达。结果发现,随培养液中三氯化铬浓度的增加,胰岛活性随之增加,凋亡率降低,但2.0μmol/L的活性有所降低,凋亡率与对照组没有显著差异(P>0.05)。低糖和高糖浓度环境中试验组与对照组的胰岛素分泌没有显著差异(P<0.05),RT-PCR发现试验组的bcl-2表达显著高于对照组(P<0.05)。2.0μmol/L CrCl3明显降低胰岛细胞的凋亡率,提高其存活率,改善胰岛功能。 |
| 关 键 词： | 三氯化铬 胰岛细胞 大鼠 胰岛素 bcl-2 |
Effects of chromic chloride on function and activity of rat pancreatic cell |
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| ZHANG Hong-xin,LI Lan-hui,YANG Hong-li,WANG Yan,LI Jun,LIAN Hai-chen,LU Shu-lan.Effects of chromic chloride on function and activity of rat pancreatic cell[J].Journal of Agricultural University of Hebei,2009,32(5). | |
| Authors: | ZHANG Hong-xin LI Lan-hui YANG Hong-li WANG Yan LI Jun LIAN Hai-chen LU Shu-lan |
| Institution: | ZHANG Hong-xin1,LI Lan-hui2,YANG Hongli1,WANG Yan1,LI Jun1,LIAN Hai-chen1,LU Shu-lan3 (1.Department of Medical Science,Hebei University,Baoding 071001,China,2.Department of Animal Science , Technology,Agricultural University of Hebei,3.Hospital of Rongjun Hebei Province,China) |
| Abstract: | To explore the mechanism and the influence of chromium chloride on the activity and insulin secretion of rat pancreatic islet cell, the activity of islet βcell was measured with MTT and trypan, and the insulin secretion was tested to evaluate the function of islet cell. The expression of antiapoptosis gene bcl-2 in islet cell was measured with RT - PCR. The results indicated that the activity of islet cell improved with the concentration of chromium chloride, but the ratio of apoptosis decreased. However, the activity of 2. 0 μmol/L chromium chloride decreased considerably, and the apoptosis ratio was not significantly different(P>0. 05). The expression of bcl-2 was significantly higher than the control group(P<0. 05). 2. 0 μmol/L chromium chloride decreased the apoptosis ratio and improved the activity of islet cell. |
| Keywords: | bcl-2 |
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