纳米处理对小麦幼胚组织培养效果及其S_1变异的影响

为了探明纳米处理对小麦幼胚组织培养效果及后代体细胞无性系变异的影响,2013-2015年以纯系龙0632和九三6529为试材,用FLM强功能陶瓷纳米器处理过的水配制培养基进行组织培养,并以正常组织培养作对照,研究了纳米处理对幼胚组培诱导频率和分化频率及其S_1主要农艺性状的变化。结果表明:经纳米处理的2份试材的平均分化率为9.3%,未经处理的为5.4%。纳米处理S_1株高的平均变异率为4.7%,穗长平均变异率为2.1%,入选株率为7.1%;未经处理的分别为0.4%,1.0%和2.7%。可见纳米处理可以提高小麦幼胚组织培养效果和后代的变异频率,有助于小麦体细胞无性系变异育种。

Effect of Nano Treatment on the Effect of Tissue Culture on Wheat Young Embryo and Mutation of S1 Generation

In order to probe into the effect of nano treatment on effect of tissue culture on wheat young embryo and mutation of S1 generation,explant medium in use nano treated purified water had been compounded,and comparison explant medium compounded by common purified water from 2013 to 2015.Frequence of induction,frequence of regeneration and mutation of important agricultural characters for S1 generation were investigated.The results showed that mean frequence of regeneration on treatmented explant medium for materials Long0632 and 93-6529 was 9.3%,and their mean frequence was 5.4% as control.The mean variable coefficient of plant hight,the mean variable coefficient of spike longth and the rate of select on treatmented explant medium for materials Long0632 and 93-6529 were 4.7%,2.1% and 7.1% respectively,and their mean variable coefficient of plant hight,mean variable coefficient of spike longth and rate of select were 0.4%,1.0% and 2.7% respectively as control.The nano treatmentcould enhance effect of tissue culture for wheat young mebryo and mutations of progeny,also can help breeding of wheat somatic mutation.