核桃楸花粉活力测定方法比较

为建立核桃楸高效快速的花粉活力测定方法,分别采用了TCC染色法、I2-KI染色法和固体培养基萌发法对核桃楸花粉活力进行了测定比较。结果表明:采用TCC染色法和I2-KI染色法染色后花粉的不易辨识,测定结果偏低,不适合作为核桃楸花粉活力的测定方法;固体培养基萌发法辨识度高,萌发率高,适合作为核桃楸花粉活力的测定方法。

Comparison on Juglans mandshurica Pollen Viability Determination Method

In order to find out the quicker and easier methods to determination the pollen viability of Juglans mandshurica,the pollen viability were detected by methods of I-KI,T TC and in vitro germination.According to the results methods,it was difficult to determine the pollen viability by the staining with TTC and I2-KI,and in vitro germination could be used to detect the pollen viability.