| 枇杷属植物基因组DNA提取方法的改进及其应用 |
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| 引用本文： | 刘月学,杨向晖,林顺权,胡桂兵,刘成明.枇杷属植物基因组DNA提取方法的改进及其应用[J].果树学报,2005,22(2):182-185. |
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| 作者姓名： | 刘月学 杨向晖 林顺权 胡桂兵 刘成明 |
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| 作者单位： | 华南农业大学园艺生物技术研究所,广州,510642 |
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| 基金项目： | 教育部骨干教师资助计划
广东省农业攻关计划项目(2004B20901001)。 |
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| 摘 要： | 以枇杷属的普通枇杷和多种野生枇杷的叶片为材料,对枇杷的DNA提取进行了研究。针对枇杷富含多酚类 等次生物质的特点对枇杷DNA的提取步骤进行了改良处理:采用CTAB-蛋白酶K法加重复抽提,去除枇杷材料中 的相关次生物质,获得了高质量的DNA。所提取的DNA完全能满足PCR、RAPD、AFLP等一些分子生物学实验要 求。
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| 关 键 词： | 枇杷属 DNA提取 |
| 文章编号： | 1009-9980(2005)02-182-04 |
| 修稿时间： | 2004年6月21日 |
An improved procedure for nuclear DNA isolation from Eriobotrya plants and its application |
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| LIU Yue-xue,YANG Xiang-hui,LIN Shun-quan,HU Gui-bing,LIU Cheng-ming.An improved procedure for nuclear DNA isolation from Eriobotrya plants and its application[J].Journal of Fruit Science,2005,22(2):182-185. |
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| Authors: | LIU Yue-xue YANG Xiang-hui LIN Shun-quan HU Gui-bing LIU Cheng-ming |
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| Abstract: | DNA extraction from leaf of loquat cultivars and several Eriobotrya species was carried out. Because loquat is rich in secondary substances such as hydroxybenzene, a series of improved treatments were used in the progress of the nucleic acid extraction. Genomic DNA was extracted from loquat species by CTAB-Proteinase K method and the extracted DNA is suitable for a series of biotechnological researches such as PCR, RAPD, AFLP etc. |
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| Keywords: | Eriobotrya Lindl DNA extraction |
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