| E.tenella吉林株单卵囊的分离及PCR鉴定(英文) |
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| 引用本文： | 杨桂连,李建华,张西臣,赵权,宫鹏涛,蔡亚南,张国才. E.tenella吉林株单卵囊的分离及PCR鉴定(英文)[J]. （《农业科学与技术》）编辑部, 2009, 0(3) |
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| 作者姓名： | 杨桂连 李建华 张西臣 赵权 宫鹏涛 蔡亚南 张国才 |
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| 作者单位： | 吉林大学畜牧兽医学院;吉林农业大学动物科学技术学院; |
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| 基金项目： | Supported by the National Natural Science Foundation of China(30671580, 30170696)~~ |
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| 摘 要： | [Objective] In order to get a purified strain to carry out the relative molecular biology research about E.tenella. [Method] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens. At the same time, the collected oocysts were identified by PCR method. [Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%. The PCR results demonstrated that the single-oocyst strain was E.tenella. [Conclusion] The inoculation of single oocyst capsule was simple, besides, this method did not only save time but also declined inoculation difficulty, increased infection rate and provided good materials for biological research of coccidian.
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| 关 键 词： | 孢子化卵囊 单卵囊 继代增殖 球虫卵囊 基因组 胶囊 分离株 吉林 雏鸡 鉴定结果 |
Isolation of E.tenella JL Strain Single-oocyst and PCR Identification |
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| YANG Gui-lian,,LI Jian-hua,ZHANG Xi-chen,ZHAO Quan,GONG Peng-tao,CAI Ya-nan,ZHANG Guo-cai. College of Animal Science , Veterinary Medicine,Jilin University,Changchun ,. College of Animal Science , Technology,Jilin Agricultural University,Changchun. Isolation of E.tenella JL Strain Single-oocyst and PCR Identification[J]. 农业科学与技术(英文版)Agricultural Science ＆ Technology, 2009, 0(3) |
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| Authors: | YANG Gui-lian LI Jian-hua ZHANG Xi-chen ZHAO Quan GONG Peng-tao CAI Ya-nan ZHANG Guo-cai. College of Animal Science Veterinary Medicine Jilin University Changchun . College of Animal Science Technology Jilin Agricultural University Changchun |
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| Affiliation: | YANG Gui-lian1,2,LI Jian-hua1,ZHANG Xi-chen1,ZHAO Quan1,GONG Peng-tao1,CAI Ya-nan1,ZHANG Guo-cai11. College of Animal Science , Veterinary Medicine,Jilin University,Changchun 130062,2. College of Animal Science , Technology,Jilin Agricultural University,Changchun 130118 |
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| Abstract: | [Objective] In order to get a purified strain to carry out the relative molecular biology research about E.tenella. [Method] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens. At the same time, the collected oocysts were identified by PCR method. [Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%. The PCR results demonstrated that the single-oocyst strain was E.tenel... |
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| Keywords: | E.tenella Single-oocyst Isolation PCR identification |
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