| 香竹愈伤组织诱导研究 |
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| 引用本文： | 孙瑞明,王娟,陈芳,彭桂莎,杨宇明,徐田.香竹愈伤组织诱导研究[J].福建林学院学报,2013,33(1):48-51. |
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| 作者姓名： | 孙瑞明 王娟 陈芳 彭桂莎 杨宇明 徐田 |
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| 作者单位： | 1. 西南林业大学,国家林业局西南生物多样性保育重点实验室,云南昆明 650224
2. 云南省林业科学院,云南昆明,650204 |
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| 基金项目： | 云南省自然科学基金资助项目(2009CD073);国家林业局“948”技术引进基金资助项目(2008-4-30);云南省中青年学术技术带头人后备人才培养基金资助项目(2010CI016) |
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| 摘 要： | 以香竹种子为培养材料进行愈伤组织的诱导和增殖,分析了不同激素组合对愈伤组织诱导和增殖的影响,并比较了香竹的种子、无菌幼根、无菌幼茎和无菌幼叶4种不同外植体诱导愈伤组织生成的效果。结果表明:香竹种子诱导愈伤组织较合适的培养基为MS+3.0 mg.L-12,4-D+0.5 mg.L-16-BA;香竹增殖愈伤组织较适合的培养基为MS+1.0 mg.L-12,4-D+1.0 mg.L-16-BA+0.5 mg.L-1KT;无菌幼根的根冠部位比幼茎和幼叶更易产生愈伤组织,而种子作为外植体效果最佳。
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| 关 键 词： | 香竹 愈伤组织 诱导 增殖 |
Study on callus inducement of Chimonocalamus delicates |
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| SUN Rui-ming
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WANG Juan
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CHEN Fang
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PENG Gui-sha
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YANG Yu-ming
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XU Tian.Study on callus inducement of Chimonocalamus delicates[J].Journal of Fujian College of Forestry,2013,33(1):48-51. |
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| Authors: | SUN Rui-ming WANG Juan CHEN Fang PENG Gui-sha YANG Yu-ming XU Tian |
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| Institution: | 1.Southwest Forestry University,Southwest Biodiversity Conservation Key Laboratory of State Forestry Administration, Kunming,Yunnan 650224,China;2.Yunnan Academy of Forestry,Kunming,Yunnan 650204,China) |
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| Abstract: | Based on murashige and skoog(MS) medium,induction and proliferation of callus under different concentration of 2,4-D,KT,and 6-BA were used for the evaluation of the effect of callus.Seeds,sterile roots,sterile stems and sterile leaves were used as explants for evaluation of the callus induction efficacy of Chimonocalamus delicatus.The results showed that MS+3.0 mg·L-1 2,4-D+0.5 mg·L-1 6-BA was an optimum medium for callus inducing with different conditions.1.0 mg·L-1 2,4-D coordinated with 1.0 mg·L-1 6-BA and 0.5 mg·L-1 KT in MS,were more suitable for callus proliferation of C.delicatus.The root cap could be easier to induce callus formation than stem and leaves.However,seeds are the most proper explants to induce the callus formation. |
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| Keywords: | Chimonocalamus delicatus callus induction proliferation |
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