|
|||||
|
|
| 小麦新种质YW243抗条锈病新基因的AFLP标记 | |
| 引用本文： | 张增艳,曾祥艳,林志珊,陈 孝,刘朝辉,中村和弘,辛志勇,吉田久. 小麦新种质YW243抗条锈病新基因的AFLP标记[J]. 中国农学通报, 2005, 21(12): 56-56 |
| 作者姓名： | 张增艳 曾祥艳 林志珊 陈 孝 刘朝辉 中村和弘 辛志勇 吉田久 |
| 作者单位： | 1. 中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程,农业部作物遗传育种重点开放实验室,北京,100081 2. 日本东北国家农业研究中心,岩手,020-0198,日本 3. 日本国际协力事业团 |
| 基金项目： | 国家“863”计划课题（2002AA207003）,日本国际协力事业团. |
| 摘 要： | 小麦新种质YW243抗条锈病新基因对条锈菌具有较宽抗谱，对26个不同毒性谱的条锈菌菌系免疫到近免疫，与已知基因系抗病谱不同，可能为新的基因或基因组合。遗传分析表明，YW243对条锈菌条中31号小种的抗性由显性单基因YrX控制。通过BSA法对1056对MseI/EcoRI AFLP引物进行筛选，结果表明7对引物可扩增出多态性片段，通过对F2群体单株遗传连锁分析和作图软件分析，结果表明，2个AFLP 标记M54E63-700、M54E64-699与YrX连锁较紧密、遗传距离为9.27 cM，为该基因的精细作图和分子标记辅助育种打下了一定基础。 |
| 关 键 词： | 小麦 条锈病 抗性基因 分子标记 AFLP |
| 收稿时间： | 2005-07-15 |
| 修稿时间： | 2005-07-152005-07-20 |
AFLP Markers Linked with a Stripe Rust Resistance Gene of a New Wheat Germplasm YW243 |
|
| Zhang Zengyan,Zeng Xiangyan,Lin Zhishan,Chen Xiao,Liu Chaohui,Kazuhiro Nakamur,Xin Zhiyong,Hisashi Yoshida. AFLP Markers Linked with a Stripe Rust Resistance Gene of a New Wheat Germplasm YW243[J]. Chinese Agricultural Science Bulletin, 2005, 21(12): 56-56 | |
| Authors: | Zhang Zengyan Zeng Xiangyan Lin Zhishan Chen Xiao Liu Chaohui Kazuhiro Nakamur Xin Zhiyong Hisashi Yoshida |
| Affiliation: | 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Genc Resources and Genetic Improvement, Key Lab. of Crop Genetic and Breeding, Ministry of Agriculture. Beijing 100081, China; 2National Agricultural Research Center for Tohoku Region, Iwate 020-0198, Japan; 3Japan International Cooperation Agency |
| Abstract: | A new wheat germplasm YW243 has a wild resistance scope against 26 different isolations of Puccinia striiformis west.tritici. The new wheat germplasm YW243 contains a new resistance gene against stripe rust, tentatively designated YrX, which was located on the long arm of chromosome 2B of wheat. In the study, the resistance pattern of a F2 population (202 individuals) derived from the cross of YW243 and Chinese Spring was analyzed through inoculation with No.31 isolate of Puccinia striiformis west. tritici. The response patterns showed that the R/S segregation ratio fits 3/1, suggesting that the gene of interest being a single dominant gene. AFLP markers were then used to search for genetically linked markers to the target gene. Using JoinMap V3.0 with Kosambi's function and other options left at default values, two AFLP markers M54E63-700 and M54E654-699 were identified to be tightly linked to YrX, with genetic distances of 9.27cM, at same position. |
| Keywords: | Wheat Stripe rust Resistance gene Molecular marker AFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国农学通报》浏览原始摘要信息 | |
| 点击此处可从《中国农学通报》下载全文 | |