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| 小麦细胞分裂素氧化酶基因Tackx3参与穗粒数的形成 | |
| 引用本文： | 常成,张海萍,张秀英,闫长生,肖世和. 小麦细胞分裂素氧化酶基因Tackx3参与穗粒数的形成[J]. 分子植物育种, 2009, 7(2) |
| 作者姓名： | 常成 张海萍 张秀英 闫长生 肖世和 |
| 作者单位： | 1. 中国农业科学院作物科学研究所,国家小麦改良中心,国家农作物基因资源与基因改良重大科学工程,北京,100081;安徽农业大学农学院,合肥,230036 2. 中国农业科学院作物科学研究所,国家小麦改良中心,国家农作物基因资源与基因改良重大科学工程,北京,100081 |
| 基金项目： | 国家科技支撑计划，农业部农业结构调整重大技术研究专项项目 |
| 摘 要： | 细胞分裂素氧化酶基因与禾本科作物的穗粒数密切相关.本文根据已报道的小麦细胞分裂素氧化酶(cytokinin oxidase,CKX)基因EST序列在GenBank中搜索更新的EST序列,组装后设计特异引物在豫麦8679/红芒春31重组自交系群体(recombined inbreed lines,RILs)中鉴定与小麦穗粒数相关的CKX基因.结果表明,Tackx3基因在RILs群体中表现较好的多态性,并与小麦穗粒数相关.具有"A"型Tackx3等位基因的家系其穗粒数大都分布在较低的范围内(穗粒数15～60),平均值为55:而具有"B"型Tackx3等位基因的家系其穗粒数大都分布在较高的范围内(61～95),平均值为65,穗粒数均值T测验达极显著水平,说明Tackx3基因等位变异与小麦穗粒数形成关系密切. |
| 关 键 词： | 小麦 穗粒数 细胞分裂素氧化酶 |
A Cytokinin Oxidase Gene, Tackx3, Involved in Forming Grains per Spike in Common Wheat |
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| Chang Cheng,Zhang Haiping,Zhang Xiuying,Yan Changsheng,Xiao Shihe. A Cytokinin Oxidase Gene, Tackx3, Involved in Forming Grains per Spike in Common Wheat[J]. Molecular Plant Breeding, 2009, 7(2) | |
| Authors: | Chang Cheng Zhang Haiping Zhang Xiuying Yan Changsheng Xiao Shihe |
| Abstract: | A tight relationship occurred between cytokinin oxidase gene (CKX) and kernel per spike (KPS) of gramineous crops, such as rice and maize. In our study, many new CKX ESTs of wheat were screened based on the ESTs deposited in GenBank. All ESTs were assembled using DNAMAN software and fell into sixteen CKX genes, respectively. Twenty four pairs of primer from the sixteen genes were used for identifying allelic variations associated with KPS in recombined inbred lines (RILs) population from a cross of Yumai867... |
| Keywords: | Tackx3 |
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