| 两种固相pH梯度胶条对桃果实蛋白质双向电泳的影响 |
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| 引用本文： | 王一鸣,胡昊,王有年,李奕松,刘悦萍.两种固相pH梯度胶条对桃果实蛋白质双向电泳的影响[J].北京农学院学报,2007,22(2):13-16. |
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| 作者姓名： | 王一鸣 胡昊 王有年 李奕松 刘悦萍 |
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| 作者单位： | 北京农学院,植物科学技术系,北京,102206;新疆农业大学,园艺学院,乌鲁木齐,830052;北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206;农业应用新技术北京市重点实验室,北京,102206;北京农学院,生物技术系 |
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| 基金项目： | 北京市自然科学基金重点项目(6071001),北京市科委区县专项资金项目(2006),北京市教委平台建设项目(2006),北京市都市农业学科群项目(XK100190553),果树生态安全创新团队资助项目 |
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| 摘 要： | 为完善用于蛋白质组分析的双向电泳体系,以"久保"桃中果皮为试材,比较pH 5.0～8.0与pH 3.0～10.0线性范围的固相pH梯度(immobilized pH gradients,IPG)胶条对蛋白质双向电泳的影响。结果表明:采用pH 5.0～8.0的17 cm胶条,90μg蛋白上样量,等电聚焦(IEF)电泳后,进行十二烷基磺酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE),银染色得出的双向电泳图有蛋白点(1 318±125)个,图谱背景清晰效果较好,适用于桃中果皮蛋白质组分析。
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| 关 键 词： | 桃 双向电泳 蛋白质 蛋白质组 |
| 文章编号： | 1002-3186（2007）02-0013-04 |
| 修稿时间： | 2007-01-28 |
Effects of Two Immobilized pH Gradients Strips on Protein Two-Dimensional Electrophoresis of Peach Mesocarp |
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| WANG Yi-ming,HU Hao,WANG You-nian,LI Yi-song,LIU Yue-ping.Effects of Two Immobilized pH Gradients Strips on Protein Two-Dimensional Electrophoresis of Peach Mesocarp[J].Journal of Beijing Agricultural College,2007,22(2):13-16. |
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| Authors: | WANG Yi-ming HU Hao WANG You-nian LI Yi-song LIU Yue-ping |
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| Institution: | 1. Department of Plant Science and Technology, Beijing University of Agriculture, Beljlng 102206, China; 2. College of Horticulture, Xinjiang Agricultural University, Urumehi 830052, China; 3. Department of Bioteehnology, Beijing University of Agrieulture,Beijing 102206,China; 4. Key Laboratory of New Technology in Agricultural Applieation of Beijing, Beijing 102206, China |
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| Abstract: | Peach(Prunus persica L.cv.Okuba) mesocarp was adopted in this experiment to consummate the protocol of two-dimensional electrophoresis for proteomic analysis and compare the effect of two-dimensional electrophoresis(2-DE) maps that were involved with immobilized pH gradients(IPG) strips with the linear range of pH5.0～8.0 and pH3.0～10.0.Approximately 90 μg of protein was loaded on 17 cm IPG strip which extension of hydrogen ion exponent was 5.0～8.0,after isoelectric focusing(IEF) completed sodium dodecyl sulfatepolyacrylamide gel electrophoresis(SDS-PAGE) was processed.The two-dimensional gel stained by silver contained 1318±125 protein spots and had a clear background on the map.The results demonstrated that this protocol was suitable for proteomic analysis of peach mesocarp. |
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| Keywords: | Prunus persica two-dimensional electrophoresis protein proteome |
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